Practice and Theory of Enzyme Immunoassays ( Volume 15 )

Publication series ：Volume 15

Author： Tijssen P.

Publisher： Elsevier Science‎

Publication year： 1985

E-ISBN: 9780080858845

P-ISBN(Paperback): 9780444806345

P-ISBN(Hardback): 9780444806345

Subject： Q55 enzyme

Language： ENG

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Description

Enzyme immunoassays have developed into a powerful assay technology, transcending several discipline boundaries, extensively applied as a tool in fields other than enzymology and immunology. This volume reflects the rapid progress in the applications of this technique, providing a basic understanding of these techniques and a practical guideline for the choice and experimental detail.

Chapter

Front Cover

pp.： 1 – 4

Practice and Theory of Enzyme Immunoassays

pp.： 4 – 5

pp.： 5 – 6

Preface

pp.： 6 – 8

Acknowledgements

pp.： 8 – 10

Contents

pp.： 10 – 22

List of abbreviations

pp.： 22 – 28

Chapter 1. Introduction

pp.： 28 – 36

Chapter 2. Outline of the strategies for enzyme immunoassays

pp.： 36 – 48

Chapter 3. Non-immunologic molecular recognition systems used in immunoassays

pp.： 48 – 66

Chapter 4. The nature of immunogens, antigens and haptens

pp.： 66 – 70

Chapter 5. Production of antibodies

pp.： 70 – 106

Chapter 6. The nature and structure of antibodies

pp.： 106 – 122

Chapter 7. Purification of immunoglobulins and preparation of Fab fragments

pp.： 122 – 150

Chapter 8. Kinetics and nature of antibody–antigen interactions

pp.： 150 – 178

Chapter 9. The nature of enzyme activity in immunoassays

pp.： 178 – 200

Chapter 10. Properties and preparation of enzymes used in enzyme immunoassays

pp.： 200 – 248

Chapter 11. Preparation of enzyme–antibody or other enzyme–macromolecule conjugates

pp.： 248 – 306

Chapter 12. Conjugation of haptens

pp.： 306 – 324

Chapter 13. The immobilization of immunoreactants on solid phases

pp.： 324 – 356

Chapter 14. Quantitative enzyme immunoassay techniques

pp.： 356 – 412

Chapter 15. Processing of data and reporting of results of enzyme immunoassays

pp.： 412 – 450

Chapter 16. Localization and identification of electrophoretically separated antigens after their transfer from gel to membrane

pp.： 450 – 476

Chapter 17. Enzyme immunohistochemistry (EIH) in light and electron microscopy

pp.： 476 – 528

Chapter 18. Potential toxic hazards associated with enzyme immunoassays

pp.： 528 – 532

References

pp.： 532 – 568

Subject Index

pp.： 568 – 578

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