Monoclonal Antibody Technology: The Production and Characterization of Rodent and Human Hybridomas ：The Production and Characterization of Rodent and Human Hybridomas ( Volume 13 )

Publication subTitle ：The Production and Characterization of Rodent and Human Hybridomas

Publication series ：Volume 13

Author： Campbell   A. M.  

Publisher： Elsevier Science‎

Publication year： 2000

E-ISBN: 9780080858821

P-ISBN(Paperback): 9780444805928

P-ISBN(Hardback):  9780444805928

Subject： Q754 gene mutation principle and structure of molecule

Language： ENG

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Description

This volume contains detailed, comprehensive advice on rat, mouse and human hybridoma production. It begins with a general introduction, then describes the practical applications of the technology with photographs and protocols for everything from animal dissection to epitope analysis of antigens.

Chapter

Front Cover

pp.：  1 – 4

Monoclonal Antibody Technology

pp.：  4 – 5

Copyright Page

pp.：  5 – 10

Preface

pp.：  6 – 8

Abbreviations

pp.：  8 – 16

Acknowledgements

pp.：  8 – 8

Contents

pp.：  10 – 6

Chapter 1. General properties and applications of monoclonal antibodies

pp.：  16 – 48

Chapter 2. Assay techniques

pp.：  48 – 81

Chapter 3. Selection of animals and cell lines

pp.：  81 – 101

Chapter 4. Immunisation

pp.：  101 – 116

Chapter 5. Cell culture requirements for hybridomas

pp.：  116 – 135

Chapter 6. Fusion procedures

pp.：  135 – 150

Chupter 7. Transformation

pp.：  150 – 166

Chapter 8. Selection and cloning

pp.：  166 – 181

Chapter 9. Antibody production and purification

pp.：  181 – 201

Chapter 10. Characterisation of monoclonal antibodies

pp.：  201 – 231

Appendix 1. Animal handling techniques

pp.：  231 – 247

Appendix 2. Addresses of suppliers and manufacturers

pp.：  247 – 250

Appendix 3. Protocols for polyacrylamide gel electrophoresis (PAGE)

pp.：  250 – 258

References

pp.：  258 – 270

Addendum

pp.：  270 – 275

Subject Index

pp.：  275 – 282

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