Lectins: Analytical Technologies :Analytical Technologies

Publication subTitle :Analytical Technologies

Author: Nilsson   Carol L.  

Publisher: Elsevier Science‎

Publication year: 2011

E-ISBN: 9780080548661

P-ISBN(Paperback): 9780444530776

P-ISBN(Hardback):  9780444530776

Subject: N3 Natural Science Research Methods;O62 Organic Chemistry;O65 Analytical Chemistry

Language: ENG

Access to resources Favorite

Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.

Description

Lectins: Analytical Technologies covers both analytical and biological aspects of lectins (functional carbohydrate (complex sugar) recognition proteins) and provides researchers in the field with a resource containing background information and 'look-up' tables detailing lectin specificity and structures. Also included are methods and practical tips for designing new lectins from existing non-lectin proteins, automated approaches to lectin proteomics and high resolution mass spectrometry techniques.



This book will be of interest to both novice and advanced researchers in biomedical, analytical and pharmaceutical fields who are involved in the study of lectin structures or who utilize lectins as analytical tools. The study of lectins and their employment in analytical settings spans a range of fields including:
* Crystallography and lectin structure databases
* Carbohydrate microarrays for lectin characterization and glycotope identification
* Proteomic approaches to the functional identification of bacterial adhesins
* Generation of lectins from enzymes
* Probing cell-surface lectins with neoglycoconjugates

* Reviews up-to-date techniques, including practical hints for laboratory work
* Provides overview of lectin e-resources and several color illustrations
* Includes a 'look-up' table detailing lectin specificity

Chapter

Front cover

pp.:  1 – 4

Copyright page

pp.:  5 – 6

Table of Contents

pp.:  6 – 8

Preface

pp.:  8 – 10

List of Contributors

pp.:  10 – 16

Chapter 2. Crystallography and Lectin Structure Database

pp.:  30 – 66

Chapter 3. NMR Investigations of Lectin–Carbohydrate Interactions

pp.:  66 – 90

Chapter 4. Applications of Isothermal Titration Calorimetry to Lectin–Carbohydrate Interactions

pp.:  90 – 118

Chapter 5. Bioanalytical Studies Based on Lectin–Carbohydrate Interactions Measured by Ellipsometry and Surface Plasmon Resonance Techniques

pp.:  118 – 144

Chapter 6. Use of Natural and Synthetic Oligosaccharide, Neoglycolipid and Glycolipid Libraries in Defining Lectins from Pathogens

pp.:  144 – 182

Chapter 7. Carbohydrate Microarrays for Lectin Characterization and Glyco-Epitope Identification

pp.:  182 – 208

Chapter 8. Glycoproteomics Based on Lectin Affinity Chromatographic Selection of Glycoforms

pp.:  208 – 228

Chapter 9. Miniaturized Lectin Affinity Chromatography

pp.:  228 – 254

Chapter 10. Frontal Affinity Chromatography: Systematization for Quantitative Interaction Analysis Between Lectins and Glycans

pp.:  254 – 282

Chapter 11. Technical Aspects of Glycoprotein Enrichment

pp.:  282 – 314

Chapter 12. Proteomic Techniques for Functional Identification of Bacterial Adhesins

pp.:  314 – 342

Chapter 13. The Use of Lectins in Bioaffinity MALDI Probes

pp.:  342 – 358

Chapter 14. Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry for Lectin Analysis

pp.:  358 – 388

Chapter 15. Catalytically Inactive Endoglycosidases as Microbial Diagnostic Reagents: Chitinases and Lysozymes as Fungal and Bacterial Capture/Label Agents

pp.:  388 – 400

Chapter 16. Generation of Lectins from Enzymes: Use of Inactive Endosialidase for Polysialic Acid Detection

pp.:  400 – 412

Chapter 17. Probing Cell Surface Lectins with Neoglycoconjugates

pp.:  412 – 432

Appendix 1. Table of Lectin Affinities

pp.:  432 – 442

Appendix 2. List of Abbreviations

pp.:  442 – 450

Subject Index

pp.:  450 – 458

The users who browse this book also browse


No browse record.