Molecular Protocols in Transfusion Medicine
Author： Denomme Gregory A.;Rios Maria;Reid Marion E.
Publisher： Elsevier Science
Publication year： 2000
E-ISBN: 9780080536903
P-ISBN(Paperback): 9780122093708
P-ISBN(Hardback): 9780122093708
Subject： Q7 Molecular Biology;R3 Basic Medical;R392 medical immunology
Language： ENG
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Description
This essential methods manual for immunohematologists (or hematologists and immunohematologists) provides information on genes that encode antigens on red blood cells, platelets and neutrophils. The book begins by covering general concepts in molecular biology and specific protocols such as DNA preparation, PCR-RFLP and allele-specific PCR. Information on the erythrocyte, platelet and neutrophil antigen systems and the molecular basis of polymorphisms are presented clearly in a gene facts sheet format. Database accession numbers and useful adjuncts such as Request forms, worksheets for PCR/enzyme digests also serve to benefit the user. The information is clearly presented and easily accessible and is complemented by the excellent diagrams and tabular material. This book is invaluable for both new and experienced researchers in the field and other related disciplines.
- Essential for hematologists and those involoved in tissue typing and the study of human genetic polymorphisms
- Presents clearly and concisely the information on a particular variant and the technique used to detect it
- Organized by antigen and provides sequences of polymorphisms and primers
- Details the general concepts and critical information on genes, their products, and sources of relevant nucleic acids
- Includes protocols that allow investigators to set up assays with minimal effort (protocols include primers, reagents, reaction conditions, sizes of amplified products, rest
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