Molecular Biology Techniques ：An Intensive Laboratory Course

Publication subTitle ：An Intensive Laboratory Course

Author： Ream   Walt;Field   Katharine G.  

Publisher： Elsevier Science‎

Publication year： 1998

E-ISBN: 9780080536828

P-ISBN(Paperback): 9780125839907

P-ISBN(Hardback):  9780125839907

Subject： Q7 Molecular Biology

Language： ENG

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Description

This manual is designed as an intensive introduction to the various tools of molecular biology. It introduces all the basic methods of molecular biology including cloning, PCR, Southern (DNA) blotting, Northern (RNA) blotting, Western blotting, DNA sequencing, oligo-directed mutagenesis, and protein expression.

  • Provides well-tested experimental protocols for each technique
  • Lists the reagents and preparation of each experiment separately
  • Contains a complete schedule of experiments and the preparation required
  • Includes study questions at the end of each chapter

Chapter

Front Cover

pp.：  1 – 4

Molecular Biology Techniques: An Intensive Laboratory Course

pp.：  4 – 5

Copyright Page

pp.：  5 – 6

Contents

pp.：  6 – 10

Preface

pp.：  10 – 12

Course Synopsis

pp.：  12 – 28

Exercises I. DNA Preparation, Polymerase Chain Reaction, and Molecular Cloning

pp.：  28 – 82

Exercises II. Protein Expression, Purification, and Analysis

pp.：  82 – 110

Exercises III. Oligonucleotide-Directed Mutagenesis

pp.：  110 – 154

Exercises IV. DNA Sequencing

pp.：  154 – 174

Exercises V. Southern Blot Detection of DNA

pp.：  174 – 202

Exercises VI. Northern Blot Detection of mRNA

pp.：  202 – 228

Exercises VII. Protein Interaction Analysis in Yeast

pp.：  228 – 240

Index

pp.：  240 – 246

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