Description
This title employs biochemical, cell biological, and genetic approaches to study mitochondrial structure, function, and biogenesis. Also of interest are the consequences of impaired mitochondrial function on cells, tissues, and organs.
The book is full of step-by-step "how to" methods with sample results, interpretations, and pitfalls. There is a unique set of appendices that include gene catalogs, mtDNA maps, and reagents for probing respiratory chain function. Finally, there are applications of state-of-the art microarray and gene chip technologies.
- Isolation of mitochondria from commonly used cells and tissues
- Assays for mitochondrial activities, including respiration, ATP production, permeability, protein import, and interactions with the cytoskeleton
- Biochemical and optical methods for studying protein-protein interactions in mitochondria
- Approaches to studying mitochondrial replication, transcription, and translation
- Transmitochondrial technologies
- Methods in microassay data analysis
Chapter
Chapter 2. Isolation and Subfractionation of Mitochondria from the Yeast Saccharomyces cerevisiae
pp.：
56 – 72
Chapter 3. Isolation and Subfractionation of Mitochondria from Plants
pp.：
72 – 94
Chapter 4. Assessing Functional Integrity of Mitochondria in Vitro and in Vivo
pp.：
94 – 116
Chapter 5. Assaying Mitochondrial Respiratory Complex Activity in Mitochondria Isolated from Human Cells and Tissues
pp.：
116 – 138
Chapter 6. In Vivo Measurements of Respiration Control by Cytochrome c Oxidase and in Situ Analysis of Oxidative Phosphorylation
pp.：
138 – 152
Chapter 7. Assay of Mitochondrial ATP Synthesis in Animal Cells
pp.：
152 – 166
Chapter 8. Measurement of Membrane Permeability and Permeability Transition of Mitochondria
pp.：
166 – 178
Chapter 9. Assaying Actin-Binding Activity of Mitochondria in Yeast
pp.：
178 – 194
Chapter 10. Analysis and Prediction of Mitochondrial Targeting Peptides
pp.：
194 – 208
Chapter 11. Assaying Protein Import into Mitochondria
pp.：
208 – 236
Chapter 12. Analysis of Protein–Protein Interactions in Mitochondria by Coimmunoprecipitation and Chemical Cross-Linking
pp.：
236 – 250
Chapter 13. Blue-Native Gels to Isolate Protein Complexes from Mitochondria
pp.：
250 – 264
Chapter 14. Application of Electron Tomography to Mitochondrial Research
pp.：
264 – 276
Chapter 15. Epitope Tagging and Visualization of Nuclear-Encoded Mitochondrial Proteins in Yeast
pp.：
276 – 296
Chapter 16. Targeting of Green Fluorescent Protein to Mitochondria
pp.：
296 – 304
Chapter 17. Assessment of Mitochondrial Membrane Potential in Situ Using Single Potentiometric Dyes and a Novel Fluorescence Resonance Energy Transfer Technique
pp.：
304 – 330
Chapter 18. Optical Imaging Techniques (Histochemical, Immunohistochemical, and in Situ Hybridization Staining Methods) to Visualize Mitochondria
pp.：
330 – 352
Chapter 19. Visualization of Mitochondrial Movement in Yeast
pp.：
352 – 372
Chapter 20. Targeting of Reporter Molecules to Mitochondria to Measure Calcium, ATP, and pH
pp.：
372 – 400
Chapter 21. Genetic Transformation of Saccharomyces cerevisiae Mitochondria
pp.：
400 – 416
Chapter 22. Transmitochondrial Technology in Animal Cells
pp.：
416 – 432
Chapter 23. Diagnostic Assays for Defects in Mitochondrial DNA Replication and Transcription in Yeast and Human Cells
pp.：
432 – 448
Chapter 24. Analysis of Mitochondrial Translation Products in Vivo and in Organello in Yeast
pp.：
448 – 458
Chapter 25. Numerical Methods for Handling Uncertainty in Microarray Data: An Example Analyzing Perturbed Mitochondrial Function in Yeast
pp.：
458 – 472
APPENDICES
pp.：
472 – 516
Volumes in Series
pp.：
532 – 540
Mitochondria
( Volume 65 )
Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.
