Neurons: Methods and Applications for the Cell Biologist ：Methods and Applications for the Cell Biologist ( Volume 71 )

Publication subTitle ：Methods and Applications for the Cell Biologist

Publication series ：Volume 71

Author： Hollenbeck   Peter J.;Bamburg   James R.  

Publisher： Elsevier Science‎

Publication year： 2003

E-ISBN: 9780080496634

P-ISBN(Paperback): 9780123525659

P-ISBN(Hardback):  9780123525659

Subject： Q189 Neurobiology;Q2 Cytobiology

Language： ENG

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Description

Neurons: Methods and Applications for the Cell Biologist lays out numerous simple techniques for growing and carrying out experiments with many varieties of neurons. Subjects include peripheral and central neurons from vertebrate and invertebrate sources, as well as neuron-like cell lines. It also explains recent advances in our ability to introduce exogenous proteins and genes to neurons in culture. Procedures for successful protein infiltration, biolistic transfection, electroporation, and viral transgenic methods in neurons are also presented.

  • Contains culture methodology for more than a dozen types of CNS and PNS neurons
  • Includes most recent and reliable techniques from expert practitioners for specific experimental applications
  • Addresses the latest strategies for transfecting neurons

Chapter

Cover

pp.：  1 – 6

Contents

pp.：  6 – 12

Contributors

pp.：  12 – 16

Preface

pp.：  16 – 18

Chapter 1. Comparing the Properties of Neuronal Culture Systems: A Shopping Guide for the Cell Biologist

pp.：  18 – 34

Chapter 2. Growing and Working with Peripheral Neurons

pp.：  34 – 54

Chapter 3. Dissection and Culturing of Chick Ciliary Ganglion Neurons: A System Well Suited to Synaptic Study

pp.：  54 – 68

Chapter 4. The Culture of Chick Forebrain Neurons

pp.：  68 – 84

Chapter 5. Growing and Working with Spinal Motor Neurons

pp.：  84 – 106

Chapter 6. Avian Purkinje Neuronal Cultures: Extrinsic Control of Morphology by Cell Type and Glutamate

pp.：  106 – 128

Chapter 7. Culturing Hippocampal and Cortical Neurons

pp.：  128 – 146

Chapter 8. Working with Xenopus Spinal Neurons in Live Cell Culture

pp.：  146 – 174

Chapter 9. Culturing Neurons from the Snail Helisoma

pp.：  174 – 188

Chapter 10. The Tibial-1 Pioneer Pathway. An in Vivo Model for Neuronal Outgrowth and Guidance

pp.：  188 – 212

Chapter 11. Techniques to Dissect Cellular and Subcellular Function in the Drosophila Nervous System

pp.：  212 – 284

Chapter 12. PC12 Cells as a Model for Studies of Regulated Secretion in Neuronal and Endocrine Cells

pp.：  284 – 304

Chapter 13. B35 Neuroblastoma Cells: An Easily Transfected, Cultured Cell Model of Central Nervous System Neurons

pp.：  304 – 322

Chapter 14. Live-Cell Imaging of Slow Axonal Transport in Cultured Neurons

pp.：  322 – 342

Chapter 15. Making Proteins into Drugs: Assisted Delivery of Proteins and Peptides into Living Neurons

pp.：  342 – 356

Chapter 16. Tranfection of Primary Central and Peripheral Nervous System Neurons by Electroporation

pp.：  356 – 370

Chapter 17. Biolistic Transfection

pp.：  370 – 386

Chapter 18. Expression of Transgenes in Primary Neurons from Chick Peripheral and Central Nervous Systems by Retroviral Infection of Early Embryos

pp.：  386 – 404

Chapter 19. Production and Use of Replication-Deficient Adenovirus for Transgene Expression in Neurons

pp.：  404 – 434

Index

pp.：  434 – 452

Volume in Series

pp.：  452 – 460

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