Apoptosis ( Volume 66 )

Publication series ：Volume 66

Author： Wilson   Leslie;Matsudaira   Paul T.;Schwartz   Lawrence M.  

Publisher： Elsevier Science‎

Publication year： 2001

E-ISBN: 9780080496610

P-ISBN(Paperback): 9780125441650

P-ISBN(Hardback):  9780125441650

Subject： Q2 Cytobiology;Q7 Molecular Biology;Q81 Bioengineering ( Biotechnology );R74 Neurology and Psychiatry

Language： ENG

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Description

Apoptosis provides a current and comprehensive collection of methods for the study of cell death. Using a diverse range of technical approaches and model systems, the chapters in this volume cover topics from the cellular and organismal to the molecular and anatomical. The methods are illustrated with user-friendly recipes and over 100 tables, halftones, and diagrams.

  • Current methodologies for studying cell death
  • Wide range of model systems
  • Molecular, biochemical, cellular, and genetic approaches
  • Complements the original Cell Death volume
  • Up-to-date methodology for a fast moving field
  • Designed with the needs of both basic scientists and clinicians in mind
  • Authors are leaders in their respective fields

Chapter

Cover

pp.：  1 – 6

Contents

pp.：  6 – 12

Contributors

pp.：  12 – 16

Preface

pp.：  16 – 18

Chapter 1. Isolation and Assay of Caspases

pp.：  18 – 46

Chapter 2. Cloning and Analysis of Bcl-2 Family Genes

pp.：  46 – 66

Chapter 3. Flow Cytometric Analysis of Cell Shrinkage and Monovalent Ions during Apoptosis

pp.：  66 – 86

Chapter 4. Use of Flow and Laser-Scanning Cytometry in Analysis of Cell Death

pp.：  86 – 128

Chapter 5. Analysis of Protein Transglutamylation in Apoptosis

pp.：  128 – 152

Chapter 6. Analysis of Sphingomyelin and Ceramide Levels and the Enzymes Regulating their Metabolism in Response to Cell Stress

pp.：  152 – 184

Chapter 7. Cell-Free Systems to Study Apoptosis

pp.：  184 – 204

Chapter 8. Role of c-Jun N-terminal Kinase in Apoptosis

pp.：  204 – 214

Chapter 9. Methods for Studying Pro- and Antiapoptotic Genes in Nonimmortal Cells

pp.：  214 – 246

Chapter 10. Calcium Flux Measurements in Apoptosis

pp.：  246 – 264

Chapter 11. Proteinase Families and Their Inhibitors

pp.：  264 – 306

Chapter 12. Identification and Analysis of Caspase Substrates: Proteolytic Cleavage of Poly(ADP-ribose)polymerase and DNA Fragmentation Factor 45

pp.：  306 – 324

Chapter 13. Analysis of Reactive Oxygen Species in Cell Death

pp.：  324 – 338

Chapter 14. Methods for Studying Apoptosis and Phagocytosis of Apoptotic Cells in Drosophila Tissues and Cell Lines

pp.：  338 – 356

Chapter 15. Phosphatidylserine Exposure and Phagocytosis of Apoptotic Cells

pp.：  356 – 382

Chapter 16. The (Holey) Study of Mitochrondria in Apoptosis

pp.：  382 – 410

Chapter 17. In Situ Detection of Dying Cells in Normal and Pathological Tissues

pp.：  410 – 434

Chapter 18. Model Cell Lines for the Study of Apoptosis in Vitro

pp.：  434 – 454

Chapter 19. Programmed Cell Death Assays for Plants

pp.：  454 – 470

Chapter 20. Studies of Apoptosis Proteins in Yeast

pp.：  470 – 486

Chapter 21. Methods to Study Cell Death in Dictyostelium discoideum

pp.：  486 – 516

Chapter 22. Methods of Study of Tumor Necrosis Factor-Related Ligands in Apoptosis

pp.：  516 – 544

Index

pp.：  544 – 564

Volumes in Series

pp.：  564 – 571

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