Author: Print Cristin
Publisher: Oxford University Press
ISSN: 1460-2350
Source: Human Reproduction, Vol.19, Iss.10, 2004-10, pp. : 2356-2366
Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.
Abstract
BACKGROUND: Angiogenesis and vascular remodeling play critical roles in the cyclical growth and regression of endometrium. They also appear to play roles in the pathogenesis of endometriosis. METHODS and RESULTS: Supernatants were collected from cultured endometrium isolated from women with and without endometriosis. These supernatants induced endothelial cell proliferation and angiogenesis in vitro. They contained vascular endothelial growth factor (VEGF)-A, and their proliferative effects on endothelial cells were partially abrogated by a blocking anti-VEGF-A antibody. Gene array analysis showed that culture supernatants from proliferative phase endometrium, and to a lesser extent secretory phase endometrium, induced significant changes in the transcriptome of endothelial cells. We could not detect any association between endometriosis and the ability of endometrial-derived soluble factors to promote angiogenesis or to regulate the endothelial transcriptome. In addition, we could not detect any association between endometriosis and the concentration of VEGF-A in supernatants from cultured endometrium or in menstrual effluent. CONCLUSIONS: We have shown that endometrium cultured in vitro produced soluble factors, including VEGF-A, that promoted angiogenesis. Proliferative phase endometrium promoted significant endothelial cell transcriptome changes that appear overall to be pro-angiogenic. These transcriptome changes provide insight into the dynamic control of vessel structure on which both eutopic endometrium and endometriotic lesions depend.
Related content
By Gargett C.E. Lederman F.L. Lau T.M. Taylor N.H.
Human Reproduction, Vol. 14, Iss. 8, 1999-08 ,pp. :
By Gargett Caroline E. Lederman Fiona Heryanto Bambang Gambino Lara S. Rogers Peter A.W.
Human Reproduction, Vol. 16, Iss. 6, 2001-06 ,pp. :
By Möller Björn Rönnerdag Maria Wang Guimin Odlind Viveca Olovsson Matts
Human Reproduction, Vol. 20, Iss. 5, 2005-05 ,pp. :