Author: Greco Claudia
Publisher: Oxford University Press
ISSN: 1460-2423
Source: Glycobiology, Vol.14, Iss.9, 2004-09, pp. : 783-792
Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.
Abstract
Galectins are a family of beta-galactoside binding molecules involved in cell–extracellular matrix adhesion processes. Specifically, Galectin-3 (Gal-3), one of the members of this family of molecules plays a role in cell adhesion processes as well as in cell survival or apoptosis. Gal-3 was also hypothesized to represent a useful tool in tumor characterization, for example, in thyroid tumors. We report herein the results obtained by evaluating Gal-3 expression of colon cells from human adenomas and adenocarcinomas with two different methodologies: immunohistochemistry and flow cytometry of living dispersed cells. We found that (1) the expression of Gal-3 was significantly increased on the surface of cells from adenomas with respect to normal mucosa from the same patient; (2) Gal-3 ligand, 90k molecule, was increased in the blood plasma from patients with both adenomatous and adenocarcinomatous lesions; and (3) Gal-3 overexpression was not related with the presence of K-ras mutation. Altogether these results clearly indicate that the evaluation of Gal-3 expression (and of its ligand, 90k) can be of interest in the characterization of nonmalignant and malignant colon cancers.
Related content
By Lutomski Didier Fouillit Magali Bourin Philippe Mellottée Daniel Denize Nicole Pontet Michel Bladier Dominique Caron Michel Joubert-Caron Raymonde
Glycobiology, Vol. 7, Iss. 8, 1997-10 ,pp. :
By Carlsson Susanne berg Christopher T Carlsson Michael C Sundin Anders Nilsson Ulf J Smith David Cummings Richard D Almkvist Jenny Karlsson Anna Leffler Hakon
Glycobiology, Vol. 17, Iss. 6, 2007-06 ,pp. :
By Nandakumar R. Wakayama M. Nagano Y. Kawamura T. Sakai K. Moriguchi M.
Protein Expression and Purification, Vol. 15, Iss. 2, 1999-03 ,pp. :
Histochemistry and Cell Biology, Vol. 130, Iss. 3, 2008-09 ,pp. :