Continuous Axenic Culture of Free-Living Nematodes

Author: Hansen Eder L.   Cryan William S.  

Publisher: Brill

ISSN: 1875-2926

Source: Nematologica, Vol.12, Iss.1, 1966-01, pp. : 138-142

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Abstract

A new method for continuous thin film axenic culture of nematodes involving a minimum of handling permits attainment of larger populations and higher proportions of adults than is attained in test tube cultures with identical media. The increase in population may be attributable to improved physiological conditions which enhance gas exchange. These observations suggest that results obtained in test tube cultures have led to an underestimation of the nutritional adequacy of the medium.