Publisher: Oxford University Press
ISSN: 1460-2377
Source: International Immunology, Vol.10, Iss.4, 1998-04, pp. : 473-480
Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.
Abstract
Decay-accelerating factor (DAFICD55) is a glycosylphosphatidylinositol-anchored protein which is known to have signal transducing capacity and to be associated with several proteins. To determine the signal transducer in the DAF-forming complex, we purified DAF-associated proteins from Raji B cells using an anti-DAF mAb (1C6)-bound affinity column and established five mAb against them. Among these, mAb 2E12-G7(IgM/κ) reacted with a variety of intact cells, including peripheral blood mononuclear cells (PBMC), as well as cells from T and B cell lines, as shown by cytofluorimetric analyses. The Mr of 2E12-G7 antigen was estimated to be 43 kDa by surface biotinylation and immunoblotting analysis. This antigen was demonstrated in 1C6 immunoprecipitates, but not in anti-CD59 (another GP]-anchored complement regulatory factor)immunoprecipitates. Sequential treatment with 1C6 F(ab')2 and then with anti-mouse IgF(ab')2 stimulated PBMC to induce tyrosine phosphorylation on proteins of 45, 72, 78 and 100 kDa. Also, mAb cross-linked to 2E12-G7 stimulated PBMC to induce tyrosine phosphorylation on proteins of 72, 78 and 100 kDa. Furthermore, when 2E12-G7 and 1C6 immunoprecipitates were incubated with [γ32P]ATP, the main constituents detected in both were phosphorylated proteins of 26, 32 and 62 kDa. Thus, DAF-associated 2E12-G7 antigen transduces a signal, similar to the DAF molecule. Keywords:CD55, complement, decay-accelerating factor, glycosylphosphatidylinositol, phosphorylation, signal transduction
Related content
By Yoshitake Hiroshi Takeda Yuji Nitto Takeaki Sendo Fujiro Araki Yoshihiko
Immunobiology, Vol. 208, Iss. 4, 2003-12 ,pp. :
By Jubinsky P.T. Messer A. Bender J. Morris R.E. Ciraolo G.M. Witte D.P. Hawley R.G. Short M.K.
Experimental Hematology, Vol. 29, Iss. 12, 2001-12 ,pp. :
By Sendo Dai Takeda Yuji Ishikawa Hitoshi Sendo Fujiro Araki Yoshihiko
Immunobiology, Vol. 207, Iss. 3, 2003-05 ,pp. :
By Pakdeesuwan Kriangsak Muangsup Wanna U-Pratya Yaowalak Issaragrisil Surapol Wanachiwanawin Wanchai
International Journal of Hematology, Vol. 73, Iss. 1, 2001-01 ,pp. :
By Tu C.-F. Hsieh S.-L. Lee J.-M. Yang L.-L. Sato T. Lee K.-H. Weng C.-N. Mao S.J.T. Tsuji K. Lee C.-J.
Transplantation Proceedings, Vol. 32, Iss. 5, 2000-08 ,pp. :