Migration of gastric cancer cells in response to lysophosphatidic acid is mediated by LPA receptor 2

Author: Yang Dezhi   Yang Wenhua   Zhang Qian   Hu Yan   Bao Liang   Damirin Alatangaole  

Publisher: Spandidos Publications

ISSN: 1792-1074

Source: Oncology Letters, Vol.5, Iss.3, 2013-03, pp. : 1048-1052

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Abstract

Lysophosphatidic acid (LPA), a natural phospholipid, is able to modulate diverse cellular responses through LPA receptors (LPARs). Several studies have reported that LPAR2 gene expression is increased in a variety of cancer cells, suggesting that LPAR2 is involved in gastric cancer. The present study investigated the expression profiles of the LPAR and involvement of the receptor subtypes in the LPAinduced migration of gastric cancer cells using cell migration assays, RNA interference, quantitative realtime PCR and western blotting. LPAR2 was observed to be highly expressed in SGC7901 cells, a human gastric cancer cell line, while LPAR1 and LPAR3 were not. Transient transfection with LPAR2 siRNA was observed to reduce LPAR2 mRNA in SGC7901 cells and eliminate the LPAinduced cell migration. It was also observed that LPAinduced SGC7901 cell migration was inhibited by the inhibitor for Gq/11 protein and p38. The results suggest that the LPAR2/Gq/11/p38 pathway regulates LPAinduced SGC7901 cell migration. The present findings suggest that LPAR2 may be a potential target for the clinical treatment of gastric cancer.

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