Author： Yamamoto H. Yoshikawa O. Nakatani M. Tsuji F. Maeda T.
Publisher： Society for Applied Spectroscopy
ISSN： 0003-7028
Source： Applied Spectroscopy, Vol.45, Iss.7, 1991-08, pp. : 1166-1170
Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.
Abstract
Direct measurement by Fourier transform near-infrared (FT-NIR) spectrometry on thin-layer chromatographic plates is described. Zones separated on a high-performance thin-layer chromatographic plate fixed on the sample stage of a microscope linked with an FT-NIR spectrometer were scanned with a narrow beam of light (0.4-mm square). The NIR chromatogram and spectra of the separated zones were then obtained by the diffuse transmission method. Three kinds of phospholipids that have essentially no absorption bands in the ultraviolet and visible regions but that do have native absorption bands in the NIR region were used to evaluate the usefulness of this method. The limit of detection for phosphatidylcholine was under 1 μg, and the correlation coefficient of the calibration curve obtained from derivative spectra was 0.998 for amounts from 1.25 to 10 μg.
Related content
Access to resources
Recommend
By Carlesi Serena Ricci Marilena Cucci Costanza Nasa Jacopo La Lofrumento Cristiana Picollo Marcello Becucci Maurizio
Applied Spectroscopy, Vol. 69, Iss. 7, 2015-07 ,pp. :