Author： Norman H.A. Pillai P.
Publisher： Elsevier
ISSN： 0003-2697
Source： Analytical Biochemistry, Vol.237, Iss.1, 1996-05, pp. : 30-36
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Abstract
A method is described for the determination of glutathione reductase activity (GR; EC 1.6.4.2) in plant extracts utilizing HPLC quantitation of NADP + following the reduction of glutathione disulfides. After protein incubation, fluorescence of NADP + was induced under strongly basic conditions, and the product was directly resolved from the reaction medium by isocratic reversed-phase elution on a silica-coated alumina support which took 2 min. The mobile phase was acetonitrile-water (50:50) delivered at a flow rate of 1.5 ml/min. The adduct (stable for at least 7 days) was detected fluorometrically and quantitated by direct integration of peak area.
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