Author： Steinberg T.H. White H.M. Singer V.L.
Publisher： Elsevier
ISSN： 0003-2697
Source： Analytical Biochemistry, Vol.248, Iss.1, 1997-05, pp. : 168-172
Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.
Abstract
Photography or electronic image acquisition is required to document results obtained from staining protein gels with the fluorescent SYPRO dyes. We found that, when using Polaroid type 667 or 57 instant films, the choice of optical filter combination and photographic exposure time strongly influences protein detection sensitivity limits. Ultraviolet light-blocking Kodak Wratten No. 2A and 2B gelatin filters autofluoresce when illuminated at 300 nm. The use of these filters in combination with Wratten No. 22 or 25 filters or SYPRO gel photographic filters gives rise to increased background signals, which for long photographic exposures can obscure signals due to protein bands. Surprisingly, the use of these same ultraviolet light-blocking filters enhanced the protein detection sensitivity obtained with short photographic exposures. Under the conditions tested, we found minimal differences in performance for Polaroid type 667 and 57 films.
Related content
Applications of SYPRO Orange and SYPRO Red Protein Gel Stains
By Steinberg T.H. Haugland R.P. Singer V.L.
Analytical Biochemistry, Vol. 239, Iss. 2, 1996-08 ,pp. :
Access to resources
Recommend
By Steinberg T.H. Jones L.J. Haugland R.P. Singer V.L.
Analytical Biochemistry, Vol. 239, Iss. 2, 1996-08 ,pp. :
Documentation of Negatively Stained Polyacrylamide Gels
By Bricker T.M. Green-Church K.B. Limbaugh P.A. Frankel L.K.
Analytical Biochemistry, Vol. 278, Iss. 2, 2000-02 ,pp. :
Western Blot of Proteins from Coomassie-Stained Polyacrylamide Gels
Analytical Biochemistry, Vol. 234, Iss. 1, 1996-02 ,pp. :