Author： Ammendola R. Ruocchio M.R. Chirico G. Russo L. de Felice C. Esposito F. Russo T. Cimino F.
Publisher： Elsevier
ISSN： 0003-9861
Source： Archives of Biochemistry and Biophysics, Vol.397, Iss.2, 2002-01, pp. : 253-257
Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.
Abstract
Reactive oxygen species have been implicated as possible second messengers in mitogenic signal transduction. We demonstrate that in normal fibroblasts the treatment with the two inhibitors of phagocytic NADH/NADPH oxidase prevents tyrosine phosphorylation of platelet-derived growth factor receptor upon the exposure of serum-deprived cells to growth factors. Furthermore, the inhibition of NADH/NADPH oxidase abolishes ERKs activation and p21waf1 accumulation that occurs when cells are exposed to growth factors. Finally, NADH/NADPH inhibitors prevent the p66Shc Ser-phosphorylation induced by serum and by phorbol 12-myristate-13-acetate, which suggests that the direct target(s) of reactive oxygen species is(are) located upstream from the machinery connecting growth factor receptors to Ras.
Related content
By Janiszewski1 M. Pedro1 M.d.A. Scheffer R.C.H. van Asseldonk J.-T.H. Souza L.C. da Luz P.L. Augusto O. Laurindo F.R.M.
Free Radical Biology and Medicine, Vol. 29, Iss. 9, 2000-11 ,pp. :
Access to resources
Recommend
By Morawietz H. Weber M. Rueckschloss U. Lauer N. Hacker A. Kojda G.
Biochemical and Biophysical Research Communications, Vol. 285, Iss. 5, 2001-08 ,pp. :
By Zhao Ruozhi Moghadasian Mohammed H. Shen Garry X.
Free Radical Research, Vol. 45, Iss. 9, 2011-09 ,pp. :