Author： Golitsina N.L. Kordowska J. Wang C.-.L.A. Lehrer S.S.
Publisher： Elsevier
ISSN： 0006-291X
Source： Biochemical and Biophysical Research Communications, Vol.220, Iss.2, 1996-03, pp. : 360-365
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Abstract
The interaction of calcyclin with tropomyosin and tropomyosin· actin was studied with fluorescence titrations and photo-reactive crosslinking experiments. Titrations of pyreneiodoacetamide-labeled tropomyosin alone or with actin showed binding of calcyclin to tropomyosin with muM dissociation constants when Ca 2+ was present. UV irradiation of mixtures of calcyclin and gizzard betabeta-tropomyosin labeled with benzophenone-iodoacetamide at Cys36, with or without actin, produced crosslinks between tropomyosin chains and calcyclin monomers only in the presence of Ca 2+ . These data provide direct evidence for a Ca 2+ -dependent tropomyosin-calcyclin interaction at or near Cys36 of tropomyosin and indicate that calcyclin binding to tropomyosin·actin does not cause tropomyosin dissociation.
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