Author： Misao R. Sun W-S. Iwagaki S. Fujimoto J. Tamaya T.
Publisher： Elsevier
ISSN： 0006-291X
Source： Biochemical and Biophysical Research Communications, Vol.252, Iss.2, 1998-11, pp. : 302-306
Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.
Abstract
We demonstrated the expression of various exon-deleted progesterone receptor (PR) variant mRNAs in human uterine endometrium and ovarian endometriosis using the reverse transcription-polymerase chain reaction-DNA sequencing analyses. In addition to PR wild-type mRNA, various exon-deleted PR variant mRNAs were identified in all samples analyzed. The sequence of these variants showed a perfect junction between exons surrouding the deletion area. PR wild-type, exon 6-deleted, exon 4-deleted, exon 5, 6-deleted and exon 4, 5, 6-deleted PR variant mRNAs were observed in all samples analyzed. Exon 4, 6-deleted PR mRNA was observed only in ovarian endometriosis. This is the first study to demonstrate the coexpression of various PR exon-deleted variant mRNAs with the wild-type in uterine endometrium and ovarian endometriosis. All resulting variant proteins might indicate functional diversity and modify the progestational action of wild-type PR, and thus be involved in the pathophysiology of ovarian endometriosis.
Related content
Access to resources
Recommend
Identification of differentially expressed mRNAs in human fetal liver across gestation
By Malhotra Khushbeer Luehrsen Kenneth R. Costello Lawrence L. Raich Teresa J. Sim Kim Foltz Lisa Davidson Scott Xu Hongxia Chen Audrey Yamanishi Douglas T. Lindemann Garrett W. Cain Carol A. Madlansacay Mary Rose Hashima Sandra M. Pham Thu Lan Mahoney Walt Schueler Paula A.
Nucleic Acids Research, Vol. 27, Iss. 3, 1999-02 ,pp. :