Inactivation of NF-κB Involved in Osteoblast Development through Interleukin-6

Author: deyama Y.   Takeyama S.   Suzuki K.   Yoshimura Y.   Nishikata M.   Matsumoto A.  

Publisher: Elsevier

ISSN: 0006-291X

Source: Biochemical and Biophysical Research Communications, Vol.282, Iss.5, 2001-04, pp. : 1080-1084

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Abstract

Osteoblasts undergo a process of proliferation and differentiation and are responsible for bone formation. In this study, we examined the relation between NF-κB, a key transcription factor in bone metabolism, and osteoblast maturation. NF-κB activity and expression of p50, a subunit of NF-κB, decreased during development of osteoblastic MC3T3-E1 cells. The secretion of IL-6 by osteoblast, which in combination with soluble IL-6 receptor induces conversion of fibroblasts to alkaline phosphatase-positive cells, also increased. p50 antisense oligonucleotide increased IL-6 mRNA expression. These results suggest that p50 regulates transcription of IL-6 and indirectly controls osteoblast maturation.

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