Pseudomonas aeruginosa infection in the hypoventilated middle ear: An experimental model

Author: Trinidad Almudena   Ramírez-Camacho Rafael   García-Berrocal Jose Ramón   María Verdaguer José   Vicente Javier   Pinilla María Teresa  

Publisher: Informa Healthcare

ISSN: 0001-6489

Source: Acta Oto-Laryngologica, Vol.125, Iss.3, 2005-03, pp. : 266-269

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Abstract

Conclusion This is a suitable model for studying different aspects of the pathophysiology of chronic suppurative otitis media. Objective To analyze the methodological features of an animal model of chronic suppurative otitis media induced by intratympanic inoculation of Pseudomonas aeruginosa. Material and methods Otitis media was induced by inoculation of P. aeruginosa through the inferior aspect of the rat bulla and cauterization of the Eustachian tube via a transpalatal approach. Inspection of the tympanic membrane, culturing of middle ear effusion and processing of the temporal bones for light microscopy were performed. Results Abnormal otomicroscopic findings and persistence of infection were correlated with the histopathological changes found in middle ear tissues.