Author： Veselkov A. Evstigneev M. Hernandez S. Rogova O. Veselkov D. Davies D.
Publisher： Springer Publishing Company
ISSN： 0022-4766
Source： Journal of Structural Chemistry, Vol.45, Iss.5, 2004-09, pp. : 793-799
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Abstract
1D and 2D 1H NMR spectroscopy (500 MHz) was used to study heteroassociation of the bifunctional intercalator ethidium homodimer (EBH) with a phenanthridine dye propidium iodide (PI) in an aqueous salt (0.1 mol/l NaCl). A physical model of equilibrium between various associated forms of ethidium homodimer and propidium in solution is suggested, the most probable forms being 1:1 and 1:2 complexes of PI with the EBH monomer and dimer. The chemical shifts of the EBH molecule in heterocomplexes, as well as the equilibrium constants and the thermodynamic parameters (enthalpy, entropy) of heteroassociations, were calculated from the concentration and temperature dependences of the proton chemical shifts. It is concluded that the propidium molecule is built (intercalated) into the EBH monomer and dimer to form 1:1 and 1:2 complexes mainly stabilized by dispersive and hydrophobic interactions.
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