Cloning and characterization of a human cDNA ACAD10 mapped to chromosome 12q24.1

Author： Ye Xin Ji Chaoneng Zhou Chun Zeng Li Gu Shaohua Ying Kang Xie Yi Mao Yumin

ISSN： 0301-4851

Source： Molecular Biology Reports, Vol.31, Iss.3, 2004-09, pp. : 191-195

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Abstract

Mitochondrial fatty acid &bgr;-oxidation is an important energy resource for many mammal tissues. Acyl-CoA dehydrogenases (ACADs) are a family of flavoproteins that are involved in the &bgr;-oxidation of the fatty acyl-CoA derivatives. Deficiency of these ACADs can cause metabolic disorders including muscle fatigue, hypoglycaemia, hepatic lipidosis and so on. By large scale sequencing, we identified a cDNA sequence of 3960 base pairs with a typical acyl-CoA dehydrogenase function domain. RT-PCR result shows that it is widely expressed in human tissues, especially high in liver, kidney, pancreas and spleen. It is hypothesized that this is a novel member of ACADs family.Abbreviations: ACADs – acyl-CoA dehydrogenases, FAD – flavin adenine dinucleotide, SCAD – short-chain acyl-CoA dehydrogenase, MCAD – medium-chain acyl-CoA dehydrogenase, LCAD – long-chain acyl-CoA dehydrogenase, VLCAD – very long- chain acyl-CoA dehydrogenase, IVD – isocalery-CoA dehydrogenase, SBCAD – short/branched chain acyl-CoA dehydrogenase, GCD – glutaryl- CoA dehydrogenase, ETF – electron transfer flavoprotein, ACAD8 – acyl-CoA dehydrogenase 8, ACAD9 – acyl-CoA dehydrogenase 9, ACAD10 – acyl-CoA dehydrogenase 10.