Author: Etter Jean-François Neidhart Elisabeth Bertrand Sonia Malafosse Alain Bertrand Daniel
Publisher: Springer Publishing Company
ISSN: 0393-2990
Source: European Journal of Epidemiology, Vol.20, Iss.10, 2005-10, pp. : 833-838
Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.
Abstract
The authors assessed whether collection by mail of saliva and buccal cells for genetic analysis was feasible in participants recruited through the Internet. In 2003, 14,773 visitors of a smoking cessation website were invited by e-mail to take part in the study. Salivettes (plastic vials containing a cotton roll) were mailed to participants, for collection of saliva and buccal cells. Because of limited resources, the authors stopped recruitment when 392 participants (3% of 14,733) were registered. They received 315 saliva samples back (80% of 392). Salivary cotinine was analyzed in 145 daily smokers. Cotinine concentration could be assessed in 141 samples (97%) (range 0.7–899 ng/ml, median 260 ng/ml). DNA extraction was achieved in all the 285 samples in which it was attempted. Quality of DNA was assessed by optical density measurements and by polymerase chain reaction amplification of a gene coding for the
Related content
Genetic analyses of sweet taste transduction
By Kennedy L.M. Eylam S. Poskanzer J.E. Saikku A.-R.
Food Chemistry, Vol. 60, Iss. 3, 1997-11 ,pp. :
International Public Health Journal, Vol. , Iss. , 2018-01 ,pp. :
By Barakat-Haddad Caroline Elliott Susan Eyles John Pengelly David
European Journal of Epidemiology, Vol. 24, Iss. 8, 2009-08 ,pp. :