Isolation and characterization of phenanthrene-degrading Sphingomonas paucimobilis strain ZX4

Author： Xia Ying Min Hang Rao Gang Lv Zhen-mei Liu Ji Ye Yang-fang Duan Xue-jun

ISSN： 0923-9820

Source： Biodegradation, Vol.16, Iss.5, 2005-10, pp. : 393-402

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Abstract

Phenanthrene-degrading bacterium strain ZX4 was isolated from an oil-contaminated soil, and identified as Sphingomonas paucimobilis based on 16S rDNA sequence, cellular fatty acid composition, mol% G + C and Biolog-GN tests. Besides phenanthrene, strain ZX4 could also utilize naphthalene, fluorene and other aromatic compounds. The growth on salicylic acid and catechol showed that the strain degraded phenanthrene via salicylate pathway, while the assay of catechol 2, 3-dioxygenase revealed catechol could be metabolized through meta-cleavage pathway. Three genes, including two of meta-cleavage operon genes and one of GST encoding gene were obtained. The order of genes arrangement was similar to S-type meta-pathway operons. The phylogenetic trees based on 16S rDNA sequence and meta-pathway gene both revealed that strain ZX4 is clustered with strains from genus Sphingomonas.