Homonuclear chemical shift correlation in rotating solids via RNνn symmetry-based adiabatic RF pulse schemes

Author: Riedel Kerstin   Leppert Jörg   Häfner Sabine   Ohlenschläger Oliver   Görlach Matthias   Ramachandran Ramadurai  

Publisher: Springer Publishing Company

ISSN: 0925-2738

Source: Journal of Biomolecular NMR, Vol.30, Iss.4, 2004-12, pp. : 389-395

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Abstract

The efficacy of RNνn symmetry-based adiabatic Zero-Quantum (ZQ) dipolar recoupling schemes for obtaining chemical shift correlation data at moderate magic angle spinning frequencies has been evaluated. RNnν sequences generally employ basic inversion elements that correspond to a net 180° rotation about the rotating frame x-axis. It is shown here via numerical simulations and experimental measurements that it is also possible to achieve efficient ZQ dipolar recoupling via RNnν schemes employing adiabatic pulses. Such an approach was successfully used for obtaining 13C chemical shift correlation spectra of a uniformly labelled sample of (CUG)97– a triplet repeat expansion RNA that has been implicated in the neuromuscular disease myotonic dystrophy. An analysis of the 13C sugar carbon chemical shifts suggests, in agreement with our recent 15N MAS-NMR studies, that this RNA adopts an A-helical conformation.

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