Attempts to eliminate Candidatus phytoplasma phoenicium from infected Lebanese almond varieties by tissue culture techniques combined or not with thermotherapy

Author： Chalak Lamis   Elbitar Ahmad   Rizk Reine   Choueiri Elia   Salar Pascal   Bové Joseph  

Publisher： Springer Publishing Company

ISSN： 0929-1873

Source： European Journal of Plant Pathology, Vol.112, Iss.1, 2005-05, pp. : 85-89

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Abstract

Elimination of Candidatus phytoplasma phoenicium from two infected Lebanese varieties of almond by using different tissue culture techniques is reported. Except for the oxytetracycline therapy which totally inhibited the development of explants, stem cutting cultures associated with thermotherapy, shoot tip cultures associated or not with thermotherapy, and shoot tip micrografting were all suitable, either for shoot regeneration or for elimination of phytoplasma from the two varieties. However, stem cutting culture coupled with thermotherapy seemed to be the most effective for regeneration of phytoplasma-free plantlets.

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