Studies on Interaction of Norfloxacin, Cu2+, and DNA by Spectral Methods

Author: Song Gongwu   Yan Qiujun   He Yu  

Publisher: Springer Publishing Company

ISSN: 1053-0509

Source: Journal of Fluorescence, Vol.15, Iss.5, 2005-09, pp. : 673-678

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Abstract

The interactions of norfloxacin (NFA), DNA, and Cu2+ are studied by fluorescence and UV-spectra method. According to the experimental results, it can be concluded that NFA can form a steady binary complex with Cu2+. There is a linear relationship between the Fluorescence intensity of the norfloxacin–Cu2+–DNA system and the concentration of DNA. And when the concentration of the NFA is 1.95×10−5 mol L−1, they possess a good linearity in the concentration of DNA ranged from 4.7×10−6 to 2.8×10−5 mol L−1.It is a good method due to the high sensitivity and selectivity.