Author： Yu Fengshan Gao Yongwei Cui Man Chen Fang Ding Yanbin
Publisher： Taylor & Francis Ltd
ISSN： 0003-2719
Source： Analytical Letters, Vol.41, Iss.16, 2008-01, pp. : 2933-2943
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Abstract
Spectrofluorimetry for the determination of prulifloxacin (PUFX) was developed based on the strong fluorescence of PUFX after adding fluorescence probe yttrium in buffer solution (pH = 6.80), and various factors of influencing fluorescence have been researched. Under the optimum conditions, the liner range was 2.0 × 10-8 to 9.1 × 10-6 mol L-1 and the detection limit was 8.5 × 10-9 mol L-1. The relative standard deviation was 2.1% for 11 measurements of 5.0 × 10-7 mol L-1 PUFX standard solution. The mechanism of the sensitizing effect of probe was discussed. The method was applied for the determination of PUFX in actual sample; the result obtained was satisfactory.
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