Author： Silva Vanezia Cerqueira Marcos Rodrigues Facchini Matos Maria Auxiliadora Costa Matos Renato Camargo
Publisher： Taylor & Francis Ltd
ISSN： 0003-2719
Source： Analytical Letters, Vol.42, Iss.1, 2009-01, pp. : 154-165
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Abstract
Glucose present in honey was rapidly determined by the differential amperometric method using two tubular reactors containing glucose oxidase and peroxidase. The linear dynamic range extends from 5 × 10-5 to 2 × 10-4mol L-1, at pH 7.0. At flow rate of 1.5 mL min-1 and injecting 150-µL sample volumes, a sampling frequency of the 33 determinations per hour is afforded. The reproducibility of the methods showed a relative standard deviation (RSD) < 4%. The detection limit of this method is 1.7 × 10-5 mol L-1. The samples analyses were compared with the parallel spectrophotometric determination.
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