Author： Stratis-Cullum Dimitra McMasters Sun Pellegrino Paul
Publisher： Taylor & Francis Ltd
ISSN： 0003-2719
Source： Analytical Letters, Vol.42, Iss.15, 2009-10, pp. : 2389-2402
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Abstract
A qualitative capillary electrophoresis immunoassay was developed for the first-time to evaluate aptamer binding to bacterial cells. Binding affinity of aptamers developed against a Campylobacter jejuni bacterial cell target, relative to other common food-borne pathogens was investigated and specific binding affinity was evidenced by pronounced mobility shift and peak broadening with increasing bacteria concentration for both aptamers. Little to no mobility shift was observed for food-borne pathogens, Salmonella typhirium and Escherichia coli, even when increasing concentrations 10-fold over target. These results suggest that affinity probe capillary electrophoresis could be useful for qualitative screening of aptamer candidates for bacterial cell targets.
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