Author： Han Dandan Tian Minglei Row Kyung Ho
Publisher： Taylor & Francis Ltd
ISSN： 1082-6076
Source： Journal of Liquid Chromatography & Related Technologies, Vol.32, Iss.16, 2009-01, pp. : 2407-2416
Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.
Abstract
Reversed-phase high-performance liquid chromatography (HPLC) was applied on a preparative scale to purify chlorogenic acid, caffeic acid, scoparone, and rutin from Herba Artemisiae Scopariae. By changing the different extraction solvents, procedures, and times, the optimum extraction condition was established using methanol/water (60:40, v/v) as the extraction solvent followed by ultrasonic extraction for 40 min. Extraction amounts of the four compounds were 4.37 mg/g, 0.27 mg/g, 0.56 mg/g, and 0.083 mg/g, respectively. The column with 40/63 µm C18 as the particles successfully isolated the extract by using acetonitrile/water/acetic acid (20:80:0.1, v/v/v) as the mobile phase.
Related content
Access to resources
Recommend
Analytical and Bioanalytical Chemistry, Vol. 388, Iss. 2, 2007-05 ,pp. :
By Liang Yong Hu Jiangyong Chen Hongwei Zhang Tianyou Ito Yoichiro
Journal of Liquid Chromatography & Related Technologies, Vol. 30, Iss. 4, 2007-03 ,pp. :
By Wen Lei Lin Yunliang Lv Ruimin Yan Huijiao Yu Jinqian Zhao Hengqiang Wang Xiao Wang Daijie
Molecules, Vol. 22, Iss. 5, 2017-05 ,pp. :
Access to resources
Journal of Liquid Chromatography & Related Technologies, Vol. 28, Iss. 17, 2005-10 ,pp. :