Forensic DNA Biology :A Laboratory Manual

Publication subTitle :A Laboratory Manual

Author: Elkins   Kelly M.  

Publisher: Elsevier Science‎

Publication year: 2012

E-ISBN: 9780123948335

P-ISBN(Paperback): 9780123945853

P-ISBN(Hardback):  9780123945853

Subject: D919.4 forensic medicine

Keyword: 社会学,法律

Language: ENG

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Description

DNA typing has revolutionized criminal investigations and has become a powerful tool in the identification of individuals in criminal and paternity cases. Forensic DNA Biology: A Laboratory Manual is comprised of up-to-date and practical experiments and step-by-step instructions on how to perform DNA analysis, including pipetting, microscopy and hair analysis, presumptive testing of body fluids and human DNA typing. Modern DNA typing techniques are provided, reflecting real life, where not all institutions and crime labs can afford the same equipment and software. Real case studies will be used throughout.

  • Provides practical step-by-step instruction on how to perform forensic DNA analysis
  • Includes analysis of hair, presumptive testing of body fluids, human DNA typing and statistics
  • Covers techniques such as pipetting, microscopy and DNA extraction
  • Pre- and post-lab exercises and questions assist the reader in learning the material
  • Report writing templates assure the reader learns real world crime lab procedure

Chapter

Welcome

Forensic DNA Biology: An Introduction

BIOLOGY OVERVIEW

RESTRICTION FRAGMENT LENGTH POLYMORPHISMS

POLYMERASE CHAIN REACTION

SHORT TANDEM REPEATS

SINGLE NUCLEOTIDE POLYMORPHISMS

MITOCHONDRIAL DNA

KNOWN VERSUS QUESTIONED SAMPLES

WHY STUDY FORENSIC DNA BIOLOGY?

Laboratory Safety

RULES FOR A SAFE LAB ENVIRONMENT

Reference

Avoiding Contamination Issues: Standard Laboratory Practices

Reference

Chapter 1 - Pipetting

OBJECTIVE

SAFETY

MATERIALS

BACKGROUND

PROCEDURE

QUESTIONS

GRAPHING THE DATA USING MICROSOFT EXCEL (2003)

EQUATIONS

References

Chapter 2 - Serology

OBJECTIVE

SAFETY

MATERIALS

BACKGROUND

PROCEDURE

QUESTIONS

References

Chapter 3 - Sampling Biological Evidence for DNA Extraction

OBJECTIVE

SAFETY

MATERIALS

BACKGROUND

PROCEDURE: DNA COLLECTION AND PACKAGING

QUESTIONS

References

Chapter 4 - DNA Extraction

OBJECTIVE

SAFETY

MATERIALS

RECIPES FOR BUFFER AND SOLUTION PREPARATION

BACKGROUND

PROCEDURE

QUESTION

References

Chapter 5 - Determination of Quality and Quantity of DNA Using Agarose Gel Electrophoresis

OBJECTIVE

SAFETY

MATERIALS

BACKGROUND

PROCEDURE

QUESTIONS

Reference

Chapter 6 - Determination of DNA Quality and Quantity Using UV-Vis Spectroscopy

OBJECTIVE

SAFETY

MATERIALS

BACKGROUND

PROCEDURE

QUESTIONS

References

Chapter 7 - Determination of DNA Quantity by Fluorescence Spectroscopy

OBJECTIVE

SAFETY

MATERIALS

BACKGROUND

PROCEDURE

QUESTIONS

References

Chapter 8 - Real-Time Polymerase Chain Reaction (PCR) Quantitation of DNA

OBJECTIVE

SAFETY

MATERIALS

BACKGROUND

PROCEDURE

QUESTIONS

References

Chapter 9 - Multiplex Polymerase Chain Reaction (PCR) Primer Design (in Silico)

OBJECTIVE

SAFETY

MATERIALS

BACKGROUND

PROCEDURE

QUESTIONS

References

Chapter 10 - Testing Designed Polymerase Chain Reaction (PCR) Primers in Multiplex Reactions

OBJECTIVE

SAFETY

MATERIALS

BACKGROUND

PROCEDURE

QUESTIONS

References

Chapter 11 - Multiplex Polymerase Chain Reaction (PCR) Amplification of Short Tandem Repeat (STR) Loci Using a Commercial Kit

OBJECTIVE

SAFETY

MATERIALS

BACKGROUND

PROCEDURE

QUESTIONS

References

Chapter 12 - Capillary Electrophoresis of Short Tandem Repeat (STR) Polymerase Chain Reaction (PCR) Products from a Commercial Multiplex Kit

OBJECTIVE

SAFETY

MATERIALS

BACKGROUND

PROCEDURE

QUESTION

References

Chapter 13 - Computing Random Match Probability from DNA Profile Data Using Population Databases

OBJECTIVE

SAFETY

MATERIALS

BACKGROUND

PROCEDURE

QUESTIONS

References

Chapter 14 - Mitochondrial Deoxyribonucleic Acid (mtDNA) Single Nucleotide Polymorphism (SNP) Detection

OBJECTIVE

SAFETY

MATERIALS

BACKGROUND

PROCEDURE

QUESTIONS

References

Chapter 15 - Analysis of Deoxyribonucleic Acid (DNA) Sequence Data Using BioEdit

OBJECTIVE

SAFETY

MATERIALS

BACKGROUND

PROCEDURE

QUESTIONS

References

Chapter 16 - Ribonucleic Acid (RNA) Extraction

OBJECTIVE

SAFETY

MATERIALS

BACKGROUND

PROCEDURE

QUESTION

References

Chapter 17 - Y-STR Polymerase Chain Reaction (PCR) Deoxyribonucleic acid (DNA) Amplification and Typing

OBJECTIVE

SAFETY

MATERIALS

BACKGROUND

PROCEDURE

QUESTION

References

Chapter 18 - Human Genetic Analysis: Paternity or Missing Persons Cases and Statistics

OBJECTIVE

SAFETY

MATERIALS

BACKGROUND

PROCEDURE

References

Chapter 19 - Low Copy Number Stochastic Results

OBJECTIVE

SAFETY

MATERIALS

BACKGROUND

PROCEDURE

QUESTIONS

References

Chapter 20 - Using in Silico Methods to Construct a Short-Tandem Repeat (STR) Deoxyribonucleic Acid (DNA) Sequence for Cloning

OBJECTIVE

SAFETY

MATERIALS

BACKGROUND

PROCEDURE

QUESTIONS

Reference

Chapter 21 - Deoxyribonucleic Acid (DNA) Extraction from Botanical Material and Polymerase Chain Reaction (PCR) Amplification

OBJECTIVE

SAFETY

MATERIALS

BACKGROUND

PROCEDURE

QUESTIONS

References

Chapter 22 - Social, Ethical, and Regulatory Concerns

References

Selected Forensic DNA Biology Case Studies

HISTORICAL DNA ANALYSIS

PATERNITY TESTING

DNA IN MODERN FORENSICCASES

MASS DISASTER CASES

DNA MIXTURES

MTDNA ANALYSIS

MICROBIAL DNA ANALYSIS

FORENSIC BOTANY

WILDLIFE FORENSICS

MEDICAL FORENSICS

TOUCH DNA

Index

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