Contents

Contributors

Introduction

PART 1 RNA interference in plants

Chapter 1 Hairpin RNAi in plants

1.1 Introduction

1.2 Discovery of hairpin RNAi

1.3 The molecular mechanisms of hairpin RNAi

1.4 Using hairpin RNAi in plants

1.5 Flexible hairpin RNAi methods

1.6 Applications of hairpin RNAi in plants

1.7 Summary

Chapter 2 Virus-induced gene silencing (VIGS) to study gene function in plants

2.1 Introduction

2.2 Principles

Chapter 3 The use of artificial microRNAs in targeted gene silencing

3.1 Introduction

3.2 Relevant issues in miRNA biogenesis for amiRNA efficiency

3.3 The pros and cons of using amiRNAs in gene silencing

3.4 Future directions to extend amiRNA technology

3.5 Conclusions

PART 2 RNA interference in animals

Chapter 4 RNAi in Caenorhabditis elegans

4.1 Introduction

4.2 Delivery of exogenous dsRNA

4.3 General considerations for the design of an RNAi experiment

4.4 Large-scale RNAi preparations for biochemical, proteomic and microarray analyses

4.5 Inducible and tissue-specific RNAi using hairpin transgenes

4.6 High-throughput RNAi screens in 96-well format

Chapter 5 RNAi in Drosophila

5.1 Introduction

Chapter 6 RNAi in fish and crustaceans

6.1 Introduction

6.2 RNAi in crustaceans

6.3 RNAi in fish

6.4 Conclusions

Chapter 7 RNAi in chicken embryos

7.1 Introduction

Chapter 8 RNAi in mammalian cells

8.1 Introduction

8.2 RNAi and related mechanisms

8.3 siRNAs and miRNAs: analogous guide RNAs for RNA silencing

8.4 RNAi in mammalian cells: searching for the right siRNAs

8.5 RNAi in mammalian cells: getting siRNAs into cells

8.6 RNAi in mammalian cells: verifying the specificity of RNAi experiments

8.7 Concluding remarks

Chapter 9 siRNA and shRNA design

9.1 Introduction

9.2 RNAi design methods

Index

A

B

C

D

E

F

G

H

I

L

M

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O

P

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V

W

Y

Z