Stable Isotopes in Human Nutrition :Laboratory Methods and Research Applications

Publication subTitle :Laboratory Methods and Research Applications

Author: Abrams   S.E.;Wong   W.W.  

Publisher: CABI Publishing‎

Publication year: 2003

E-ISBN: 9780851997964

P-ISBN(Hardback):  9780851996769

Subject: R151 Nutrition

Keyword: Dietics and Nutrition

Language: ENG

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Description

The use of stable isotopes in nutritional studies is now widespread, and the technique is becoming increasingly popular. Practical applications are numerous and include:calcium and iron absorption studiesstudies looking at the impacts of diet, physical activity, aging, and medical therapy and supplementation on nutrient metabolismthe measurement of energy cost of pregnancystudies on the causes of growth faltering in infantsinvestigations into childhood and adult obesity.This book is designed as a laboratory handbook of methods used to perform stable isotope studies in humans. It covers basic principles, dosage information, sample preparation procedures, analytical instrumentation, and necessary mathematical methods and provides the fundamentals to enable researchers to evaluate and establish stable isotope methods in their own laboratories.

Chapter

1 Introduction

2 Production of Stable Isotopes for Nutrition-related Research

3 The Measurement of Protein Kinetics with Stable Isotope Tracers

4 Stable Isotope Studies of Mineral Metabolism: Calcium, Magnesium and Iron

5 Evaluation of Trace Mineral Status and Bioavailability Using Stable Isotopes (Zinc, Copper, Selenium, Molybdenum)

6 Energy Utilization with Doubly Labelled Water (2H2 18O)

7 Body Composition Measurements with 2H and 18O Isotope Dilution

8 Stable Isotopes and Gas Chromatography–Mass Spectrometry in Studies of Glucose Metabolism in Children

9 Cholesterol and Other Lipid Metabolism

Index

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