Front Cover

Recent Progress of Life Science Technology in Japan

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Table of Contents

Foreword

Preface

Preface

PART I: DNA SEQUENCING TECHNOLOGIES

CHAPTER 1. TEMPERATURE-GRADIENT DNA-PROBE COLUMN CHROMATOGRAPHY: A NEW METHOD FOR DETECTION AND PURIFICATION OF PARTICULAR DNAS OR RNAS

I. INTRODUCTION

II. THERMAL STABILITY OF PROBE-SAMPLE HYBRID DUPLEX

III. TEMPERATURE-GRADIENT DNA-PROBE COLUMN CHROMATOGRAPHY

ACKNOWLEDGMENTS

REFERENCES

CHAPTER 2. Development of Automatic Technics of Purification Process of M13 Single Strand DNA and Sequence Reaction Based on Sanger Method

I. Purpose

II. Method

III. Results

IV. Results and discussion

V. General References

CHAPTER 3. Development of Electrophoresis Precast Gel for DNA Sequencing

I. INTRODUCTION

II. METHODOLOGY

III. RESULTS AND DISCUSSIONS

CHAPTER 4. REAL-TIME DNA DETECTION SYSTEMS

I. INTRODUCTION

II. Real-time β -ray detection system

III. REAL-TIME FLUORESCENCE DETECTION SYSTEM

REFERENCES

CHAPTER 5. TWO DIMENSIONAL IMAGE PROCESSING OF ELECTRON MICROGRAPHS OF tRNA THIN CRYSTALS

I. Introduction

II. Materials and Methods

III. Results and Discussion

REFERENCES

CHAPTER 6. A UNIFORM MONOLAYER SPREAD FROM AN AQUEOUS SOLUTION ON A CLEAN MERCURY SURFACE: TOWARD TWO-DIMENSIONAL CRYSTALLIZATION OF PROTEINS AND NUCLEIC ACIDS

I. INTRODUCTION

II. METHOD AND MATERIALS

III. Results

IV. DISCUSSION

ACKNOWLEDGMENTS

REFERENCES

PART II: GENE TRANSFER & EXPRESSION TECHNOLOGIES FOR CANCER RESEARCH

CHAPTER 7. ELECTRIC PULSE-MEDIATED GENE TRANSFER IN MAMMALIAN CELLS

I. INTRODUCTION

II. METHODS FOR GENE TRANSFER

III. OUR RESULTS

IV. DISCUSSION

ACKNOWLEDGMENTS

REFERENCES

CHAPTER 8. A POSITIVE REGURATORY REGION AND ITS FUNCTION IN YEAST ENO1 PROMOTER

I. INTRODUCTION

II. DETERMINATION OF A POSITIVE REGULATORY REGION OF ENO1 GENE

III. FUNCTION OF A POSITIVE REGULATORY REGION OF ENO1 GENE

ACKNOWLEDGMENTS

REFERENCES

CHAPTER 9. COOPERATION OF EXOGENOUS ONCOGENES IN CELL CULTURE

I. INTRODUCTION

II. Ad12 transforming gene mutants

III. Ad4-induced incomplete transformants

IV. Cells constitutively expressing exogenous myc gene

V. ACKNOWLEDGEMENTS

VI. REFERENCES

CHAPTER 10. COLLABORATIVE TRANSFORMATION WITH TWO ONCOGENES; myc COLLABORATING WITH V-snc IN PRIMARY CELLS AND WITH AN IMMORTALIZATION-POSITIVE SV40 MUTATED ONCOGENE IN ESTABLISHED RAT CELLS

INTRODUCTION

MATERIALS AND METHODS

RESULT

DISCUSSION

SUMMARY

ACKNOWLEDGMENT

REFERENCES

CHAPTER 11. MEDAKA, A USEFUL EXPERIMENTAL SYSTEM FOR CHEMICAL AND ENVIRONMENTAL CARCINOGENESIS

I. INTRODUCTION

II. HEPATOCARCINOGENESIS IN ORANGE-RED VARIETY

III. TUMORIGENESIS IN INBRED STRAIN MEDAKAS

IV. EXPERIMENTS WITH CULTURED CELLS OF MEDAKA

ACKNOWLEDGEMENTS

REFERENCES

Chapter 12. An Attempt to Develop An in vitro Infection System of B Lymphocyte by Bovine Leukemia Virus

I. INTRODUCTION

II. RESULTS

III. Discussion

ACKNOWLEDGEMENTS

REFERENCES

CHAPTER 13. FUNCTION OF BOVINE LEUKEMIA VIRUS (BLV) TAX AND REX GENES: In an attempt to reconstruct potent BLV enabling in vitro bovine B cell transformation

I. INTRODUCTION

II. IDENTIFICATION OF pΧBL-Ι (TAX) PROTEIN AND ITS mRNA

III. TRANSACTIVATING CAPACITY OF BLV TAX PROTEIN

IV. MULTIPLE TARGET ELEMENTS OF p38BLV-tax IN BLV-LTR

V. HOW DOES BLV TRANS-ACTIVATOR INTERACT WITH ITS TARGET ELEMENTS?

VI. DISCOVERY OF pX-II (REX) FRAME AND IDENTIFICATION OF REX PROTEINS

VII. BIOLOGICAL FUNCTIONS OF BLV REX I AND II

VIII. ATTEMPT TO RECONSTRUCT MORE EFFICIENT BLV APPLICABLE FOR IN VITRO LEUKEMOGENESIS

REFERENCES

PART III: TECHNOLOGIES FOR EXTRACTION, PURIFICATION & STRUCTURAL ANALYSIS OF CANCER-RELATED PROTEINS

CHAPTER 14. DEVELOPMENT OF NEW TECHNIQUES FOR IDENTIFICATION, PURIFICATION AND CHARACTERIZATION OF CANCER CELL-SPECIFIC PROTEINS

I. AIM OF THIS RESEARCH

II. EXPERIMENTAL DESIGN

III. RESULTS

IV. PERSPECTIVE

ACKNOWLEDGMENTS

REFERENCES

CHAPTER 15. A PROCEDURE TO ISOLATE INTRACELLULAR REDIFFERENTIATION FACTORS IN MOUSE ERYTHROLEUKEMIA CELLS

INTRODUCTION

METHODS

RESULTS

SUMMARY

ACKNOWLEDGMENT

REFERENCES

CHAPTER 16. IDENTIFICATION, CHARACTERIZATION AND PURIFICATION OF A UNIQUE ANTIGEN IN B6RV2 LEUKEMIA

I. INTRODUCTION

II. IDENTIFICATION OF A UNIQUE ANTIGEN ON B6RV2 LEUKEMIA BY CELL-MEDIATED CYTOTOXICITY

III. DETECTION OF A UNIQUE ANTIGEN ON B6RV2 LEUKEMIA BY MONOCLONAL ANTIBODY

IV. BIOCHEMICAL CHARACTERIZATION OF A UNIQUE ANTIGEN ON B6RV2 LEUKEMIA

V. PURIFICATION OF B6RV2 UNIQUE ANTIGEN

VI. CONCLUSION

REFERENCES

CHAPTER 17. Chemical Properties of Metal Ions and Carcinogenesis

Indroduction

1. Mutagenicity of Metallic Compounds

2. Carcinogenesis with Metallic Compounds

3. Carcinogenic Mechanisms of Metal Ions

REFERENCES

CHAPTER 18. STUDIES ON TECHNIQUES FOR INTRACELLULAR INTRODUCTION OF MACROMOLECULES

I. INTRODUCTION

II. EXPRESSION OF ONCOGENES

III. INTRODUCTION OF MACROMOLECULES USING LIPOSOMES

ACKNOWLEDGMENT

REFERENCES

CHAPTER 19. A NEW STRATEGY AND TACTICS FOR PROTEIN SEQUENCING

I. INTRODUCTION

II. FLUORESCENT KOSHLAND REAGENT WITH CHELATING ABILITY. SELECTIVE ISOLATION OF TRYPTOPHAN–CONTAINING PEPTIDE FRAGMENTS FROM ENZYMATIC PROTEIN DIGEST

III. A NEW FLUOROGENIC REAGENT FOR THIOL GROUP APPLICATION TO ANALYSIS OF PROTEINS

IV. OTHER TECHNOLOGIES SUPPORTING PROTEIN SEQUENCING

REFERENCES

PART IV: TECHNOLOGIES FOR ANALYSIS & SYNTHESIS OF SACCHARIDE CHAINS

CHAPTER 20. ENZYMIC ANALYSIS OF GLYCOSAMINOGLYCAN STRUCTURE

I. INTRODUCTION

II. SEPARATION OF UNSATURATED DISACCHARIDE ISOMERS

III. ENZYMIC DEGRADATION OF GLYCOSAMINOGLYCAN

IV. ENZYMIC IDENTIFICATION OF DISACCHARIDE

CHAPTER 21. SYNTHETIC STUDIES ON GLYCAN CHAINS

ACKNOWLEDGMENTS

RERERENCES

CHAPTER 22. ESTABLISHMENT AND CHARACTERIZATION OF METASTATIC ASCITES HEPATOMA VARIANTS WITH DIFFERENT ADHESIVE PROPERTIES TO SUBSTRATE IN VITRO

I. INTRODUCTION

II. SELECTION OF VARIANTS WITH DIFFERENT ADHESIVE PROPERTIES FROM RAT ASCITES HEPATOMA ΑΗ7974 IN VITRO

III. ADHESION-KINETICS AND GROWTH RATE

IV. CELLULAR CHARACTERISTICS OF 74AD AND 74FL CELLS

V. ADHESIVE POTENTIAL TO CULTURED ADULT RAT HEPATOCYTES

VI. EXPERIMENTAL METASTATIC POTENTIAL

VII. SUMMARY AND CONCLUSION

VIII. REFERENCES

CHAPTER 23. DETERMINATION AND CHARACTERIZATION OF MELANOMA ANTIGENS RECOGNIZED BY MONOCLONAL ANTIBODIES

I. INTRODUCTION

II. ANTIGEN RECOGNIZED BY M2590

III. PURIFICATION AND CHARACTERIZATION OF LAC-CER: NeuAc SIALYLTRANSFERASE

IV. ANTIGEN RECOGNIZED BY M562 AND M622 WITH MOUSE MELANOMA SPECIFIC REACTIVITY

V. CONCLUSION

REFERENCES

CHAPTER 24. Use of antitermination signals to obtain efficient expression of genes in E. coli : Host cell protein factors involved in transcription antitermination

I. INTRODUCTION

II. MOLECULAR STRUCTURE AND NUCLEOTIDE SEQUENCE OF THE nusA-infB OPERON

III. PROMOTER OF THE nusA-infB OPERON

IV. NUCLEOTIDE SEQUENCE OF THE nusB GENE AND ITS FLANKING REGION

V. CHEMICAL PROPERTIES OF NusA AND NusB proteins

VI. FREQUENCY OF CODON USAGE IN THE nus A AND nusB GENES

Acknowledgments

REFERENCES

CHAPTER 25. Automatic Selection System of Monoclonal Antibody Producing Cells

I. Introduction

II. System Function

III. System Operation

IV. Function Test

V. Conclusion

REFERENCES

Index