Research on Steroids :Proceedings of the Fourth Meeting of the International Study Group for Steroid Hormones

Publication subTitle :Proceedings of the Fourth Meeting of the International Study Group for Steroid Hormones

Author: Finkelstein   M.;Klopper   A.;Conti   C.  

Publisher: Elsevier Science‎

Publication year: 2013

E-ISBN: 9781483154039

P-ISBN(Paperback): 9780080175737

P-ISBN(Hardback):  9780080175737

Subject: Q548 steroids (sterol)

Language: ENG

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Description

Research on Steroids contains the proceedings of the Fourth Meeting of the International Study Group for Steroid Hormones. The papers explore the production, chemical characterization, and metabolism of steroid hormones and their interactions with proteins. Topics range from the action of estradiol in vitro to water-soluble metabolites of estrogens, transport of steroids by proteins, and immunological aspects of steroid hormones. The binding of steroids by tissue proteins is also discussed, along with the use of specific plasma proteins for the determination of steroids.
This volume is comprised of 38 chapters and begins with an overview of the general aspects of steroid-protein interaction, followed by a discussion on quantitative studies of steroid binding proteins; aromatization of testosterone by human placenta enzymes; relationship between plasma binding and 5α-reduction of testosterone; cortisol-binding capacity of plasma transcortin in subjects treated with various anabolic steroids; and plasma testosterone binding capacity and estrogen excretion in normal and pathological pregnancies. Subsequent chapters deal with the interaction between estrogenic and carcinogenic substances in the rat mammary gland; influence of steroid production on serum albumin in Cushing's syndrome; and nuclear estradiol binding proteins in the uterus.
This book will be of interest to biochemists, biologists, and physiologists.

Chapter

Acknowledgements

References

Chapter 1. The ''Biological Exchange Constant" of Estradiol-17)B between Plasma and Uterus in the Adult and Immature Rats

Acknowledgements

References

Chapter 2. Quantitative Studies of Steroid Binding Proteins

References

Chapter 3. Action of Oestradiol in vitro: Increase in Ribonucleic Acid Biosynthesis in Uterine Nuclei

References

Chapter 4. Aromatization of Testosterone by Human Placenta Enzymes: Role of a Protein Fraction obtained from pregnant Female Serum which binds Testosterone (T) and Oestradiol-17)B (E2)

Introduction

Isolation of a testosterone binding protein fraction

Behaviour of oestradiol toward a testosterone binding protein fraction

Influence of the isolated protein fraction on the aromatization of testosterone by placental enzymes

Results

References

Chapter 5. Stability and Nature of in vivo Binding between Testosterone, its Metabolites and Organ Proteins in Rats

References

Chapter 6. Water Soluble Metabolites of Oestrogens: A Model of Covalent Steroid Protein Binding

Acknowledgements

References

Section II: Transport of Steroids by Proteins

Short description of the main steroid binding proteins

Albumin

Transcortin

Orosomucoid

Testosterone binding protein

Changes in plasma binding protein concentrations

Physiological increase of serum binding proteins

Transcortin

17β SBP (17β OH Steroid binding protein)

Serum binding protein changes under experimental conditions

Oestrogens

Thyroxin

Pathological conditions

Transcortin

17/3 SBP (17ß hydroxy steroid binding protein)

Other typical experiments

Concluding remarks

References

Chapter 8. The Binding of Progesterone by the α1 -Acid Glycoprotein

Material and methods

Results and discussion

Acknowledgment

References

Chapter 9. Interaction of Steroids with Human Red Blood Cells

Materials and methods

Results

Discussion

Acknowledgements

References

Chapter 10. Relationship between Plasma Binding and 5a-Reduction of Testosterone

Material and Methods

Results

Discussion

Acknowledgments

References

Chapter 11. Comparative Studies on the Binding of Testosterone and 17B-Oestradiol by Serum Proteins in Normals and in Patients with Liver Cirrhosis

Acknowledgments

References

Chapter 12. Cortisol-binding Capacity of Plasma Transcortin in Subjects treated with various Anabolic Steroids

Materials and methods

Results

Discussion

Acknowledgments

References

Chapter 13. A 4S Oestradiol-Binding Protein in Uteri from Mature Rats that is Controlled by Endogenous Hormones

References

Chapter 14. Plasma Testosterone Binding Capacity and Oestrogen Excretion in Normal and Pathological Pregnancies

Materials and methods

Results

Discussion

Addendum

Acknowledgements

References

Chapter 15. Interaction between Oestrogenic and Carcinogenic Substances in the Rat Mammary Gland

References

Chapter 16. Metabolization and Binding of Oestradiol17B by Rat Ventral Prostate in vitro

References

Chapter 17. Interaction of Diethylstilbestrol with Uterine Receptor Sites

Uptake and sub-cellular distribution of diethylstilbestrol

Binding of diethylstilbestrol to uterine receptors

Effect of pH on the binding of DES-3 Η to the cytosol fraction

Interchange of protein-DES complex between the nuclear and cytosol fraction

Acknowledgements

References

Chapter 18. Biological Inactivation of Progesterone by Interaction with Corticosteroidbinding Globulin and with Albumin

Materials and methods

Results and discussion

Summary

Acknowledgements

References

Chapter 19. Effects of Clomiphene Citrate on Binding Affinity of Testosterone, Oestradiol and Cortisol in Serum of Normal Adult Men

Material and methods

Results

Discussion

References

Chapter 20. Influence of Steroid Production on Serum Albumin in Cushing's Syndrome

References

Chapter 21. Studies with Antibodies to Steroids

Preparation of antibodies to steroids

Physiological studies

The role of 17ß-estradiol and progesterone in implantation in the rat

Use of steroid antibodies to measure plasma steroids

Acknowledgement

References

Chapter 22. Neutralization of Exogenous Estradiol Activity following Active Immunization Procedures

Methods

Results

Discussion

Acknowledgements

References

Chapter 23. Antibodies to Oestrogens

Mode of coupling of steroid to peptide carrier

Purification of antibodies

Antibodies to non-steroidal oestrogens

Conclusions

Acknowledgements

References

Chapter 24. Solid Phase Radioimmunoassay of Estradiol-17i3 and Estrone

Methods

Results

Discussion

Acknowledgements

References

Chapter 25. Binding of Steroids by Tissue Proteins Steroid hormone ''receptors''

Estrogen-receptors

Receptors for other steroid hormones

References

Chapter 26. Cortisol-Binding Protein from various Tissues

References

Chapter 27. Progesterone-binding by the Soluble Fraction of Corpus Luteum from the pregnant Cow

Acknowledgements

References

Chapter 28. Effect of 12 and Sulphydryl Reagents on Binding Activity of Estrogen Receptors

Material and methods

Results

Discussion

Acknowledgements

References

Chapter 29. Nuclear Oestradiol Binding Proteins in the Uterus

A specific protein of very high affinity for oestradiol in the chromatin of calf endometrium

4-5 S receptor in rat uterus nuclei

References

Chapter 30. Oestradiol-binding and Protein Phosphorylation

References

Chapter 31. Partial Purification of an Estrophilic Protein from Calf Uterus

Material and methods

Results

References

Chapter 32. Use of Specific Plasma Proteins for the Determination of Steroids

A, The steroid-binding globulins

B. Theoretical study of the binding curve

C. Applications of the method

References

Chapter 33. Plasma Estrogen Assays Utilizing the Cytoplasmic Estrogen Binder of Rabbit Uterus

Methods

Results and discussion

References

Chapter 34. Plasma Progesterone and 17a-hydroxyprogesterone measured by a Competitive Protein Binding Technique and related to the Oestrogen Excretion and LH Level around Ovulation in the normal Menstrual Cycle

Materials and methods

Results and discussion

References

Chapter 35. ACTH-lnduced Fall in Plasma Testosterone

Material and methods

Results and comments

Acknowledgement

References

Chapter 36. A Method for the Estimation of Plasma Testosterone using a Technique depending on Competitive Protein Binding

1. Extraction

2. Defatting of plasma samples by a solubilization step

3. Thin layer chromatography

4. CPB assay of testosterone

References

Chapter 37. Study of C19O2 Steroids in Human Plasma by Competitive Protein Binding

Chapter 38. Partial Purification and Preliminary Characterization of an Estrogen Binding Protein (Estrogen Receptor) from Calf Uterus Nuclear Fraction

Summary

Experimental Procedure and Results

Discussion

Acknowledgements

References

Author Index

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