Cluster of differentiation 44- and octamer-binding transcription factor-4-positive stem-like osteosarcoma cells involved in tumor development

Author：

Publisher： Spandidos Publications

E-ISSN： 1792-1082|10|1|273-276

ISSN： 1792-1074

Source： Oncology Letters, Vol.10, Iss.1, 2015-01, pp. : 273-276

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Abstract

Osteosarcoma (OS) is an aggressive primary bone cancer that usually affects children and young adolescents. Previous studies have demonstrated the implications of a small subpopulation of cancer stem cells on treatment failure and tumor recurrence. The present study analyzed the characteristic features of the stemlike cells within the human OS55 cell line. It was identified that 2.3% of the OS55 cells were cancer stemlike side population (SP) cells. Following treatment with verapamil, the population of SP cells was reduced to 0.7%. The sphere formation assay revealed that the OS cells were able to rapidly form tumor spheres (also known as sarcospheres). Immunofluorescence analysis identified that the OS55 cells expressed the cluster of differentiation 44, octamerbinding transcription factor3/4A and Nanog stem cell surface markers. The results of the present study suggest that, as with other tumors, OS also contains a subpopulation of cancer stemlike cells, which may have important implications in cancer diagnosis and treatment.