Publisher： Spandidos Publications
E-ISSN： 1791-244X|35|4|1126-1132
ISSN： 1107-3756
Source： International Journal of Molecular Medicine, Vol.35, Iss.4, 2015-01, pp. : 1126-1132
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Abstract
Bupleurum falcatum L. is employed in oriental medicine in Korea. This root has been used for antiinflammatory, antipyretic, and antihepatotoxic effects in the treatments of common cold, fever, and hepatitis. One of major bioactive compounds of Radix Bupleuri is the saikosaponin a (SSNa). However, little is known concerning the effects of SSNa on obesity associated with a state of lowgrade inflammation. Consequently, this study was conducted to determine the inhibition of the inflammation pathway of SSNa in obesity. MTT assay was conducted for cytotoxicity and viability; nuclear and cytoplasmic fractions were extracted from adipocytes for translocation of nuclear factorκB cells (NFκB); nitric oxide (NO) production and secretion using Griess reagent; reverse transcriptionpolymerase chain reaction (RTPCR) and immunoblotting for mRNA and protein levels associated with inflammation in the hypertrophied adipocytes. The results revealed that SSNa significantly decreased the expression of tumor necrosis factorα (TNFα), interleukin (IL)1β and IL6 as proinflammatory cytokines, compared to that of nontreated control cells. Inducible nitric oxide synthase (iNOS) and cyclooxygenase2 (COX2) as inflammatory factors were reduced by treatment of these cells with SSNa and also suppressed NO production. Phosphorylation of IκBα was inhibited and translocation of NFκB was suppressed via the ERK pathway in response to SSNa treatment. In conclusion, the results demonstrated that SSNa can inhibit the expression of inflammatoryassociatied genes in hypertrophied 3T3L1 adipocytes and is a potent inhibitor of NFκB activation. Thus these results suggest that SSNa is a novel therapeutic agent against that can be used against obesityassociated inflammation.
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