MicroRNA-145 suppresses cell proliferation, invasion and migration in pancreatic cancer cells by targeting NEDD9

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E-ISSN： 1791-3004|11|6|4115-4120

ISSN： 1791-2997

Source： Molecular Medicine Reports, Vol.11, Iss.6, 2015-01, pp. : 4115-4120

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Abstract

MicroRNAs (miRNAs) represent a class of small noncoding RNAs regulating gene expression by inducing the degradation of RNA or interfering with translation. Aberrant miRNA expression has been described in several types of cancer in humans. In the present study, it was demonstrated that miR145 is downregulated in pancreatic cancer tissues and the Panc1 cell line. Restoration of miR145 inhibited cell proliferation, invasion and migration in Panc1 cells. Neural precursor cell expressed, developmentally downregulated 9 (NEDD9) has been identified as a novel potential miR145 target using bioinformatics. Using luciferase reporter constructs, it was observed that the NEDD9 3'untranslated region is the location of the direct binding site for miR145. Additionally, it was identified that miR145 is inversely correlated with NEDD9 expression in pancreatic cancer tissues and that restoration of miR145 in Panc1 cells reduced NEDD9 mRNA and protein expression accompanied by inhibition of cell proliferation, invasion and migration. In conclusion, these findings indicate that miR145 may be an effective target for pancreatic cancer therapy.