Publisher： Spandidos Publications
E-ISSN： 1792-1015|9|5|1768-1772
ISSN： 1792-0981
Source： Experimental and Therapeutic Medicine, Vol.9, Iss.5, 2015-01, pp. : 1768-1772
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Abstract
Paeoniflorin (PF) is a product derived from Paeoniae Radix and is commonly prescribed in traditional Chinese medicine. PF has been reported to exhibit neuroprotective, antiischemic, antioxidant, antiinflammatory and anticancer effects. The neuroprotective properties of PF have been demonstrated in animal models of various neuropathologies. The present study investigated the effects of PF on hydrogen peroxide (H2O2)induced apoptosis in PC12 cells, to improve the understanding of the mechanisms underlying its neuroprotective properties. The H2O2induced apoptosis of PC12 cells resulted in a reduction in the Bcell lymphoma 2 (Bcl2)/Bcl2associated X protein ratio and the activation of caspase3. PF treatment was observed to reverse the apoptotic process and to modulate the expression levels of a number of apoptosisassociated proteins. Furthermore, PF significantly mitigated the H2O2induced reduction in cell viability, in addition to scavenging reactive oxygen species and preventing the release of lactate dehydrogenase from the PC12 cells. In addition, the apoptosisassociated activation of nuclear factor (NF)κB was inhibited in the PFtreated cells, and the expression levels of tumor necrosis factor α and interleukin (IL)1β were reduced. In conclusion, the present study demonstrated that PF was able to reduce H2O2induced toxicity by blocking the activation of the neuroinflammatory factor NFκB. These results suggest that PF may be a valuable neuroprotective agent for the treatment of neurological disease and injury.
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