Publisher： Science Publishers
ISSN： 2049-9434
Source： Biomedical Reports, Vol.3, Iss.4, 2015-01, pp. : 578-582
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Abstract
The aim of the present study was to investigate the effects of advanced glycation end products (AGEs) on the expression of angiopoietin-like protein 4 (ANGPTL4) and the mechanisms of the effects in endothelial cells. Endothelial cells were incubated with various concentrations of AGEs for 24 h and the expression of ANGPTL4 was detected by quantitative polymerase chain reaction and western blot analysis. The concentration of angiotensin (Ang ) in conditioned media and cell lysates was measured by enzymelinked immunosorbent assays. Fluorescein isothiocyanatelabeled dextran filtration assays and transendothelial electrical resistance were performed to evaluate endothelial permeability. AGEs (80 µg/ml) increased the expression of ANGPTL4 and the levels of Ang (P<0.05). Incubation with AGEs also resulted in a significant increase in endothelial permeability (P<0.05). However, pretreatment with the Ang receptor blocker losartan (105 M) reduced the effects of AGEs (P<0.05). AGEs upregulated the expression of ANGPTL4 by activating a local reninangiotensin system in endothelial cells. This may be a new mechanism by which AGEs increase endothelial permeability.
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