IMMUNOLOGICAL STUDIES OF AN EXTRACELLULAR KERATINASE

Publisher: John Wiley & Sons Inc

E-ISSN: 1346-8138|10|4|327-330

ISSN: 0385-2407

Source: THE JOURNAL OF DERMATOLOGY, Vol.10, Iss.4, 1983-08, pp. : 327-330

Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.

Previous Menu Next

Abstract

ABSTRACTThe extracellular keratinase from M. canis was purified using ion exchange chromatography and gel filtration. The purified enzyme showed a single protein band on sodium dodecyl sulfate‐polyacrilamide gel electrophoresis. A molecular weight of approximately 45,000 was determined by SDS‐electrophoresis.Anti‐body directed against the purified enzyme from M. canis was obtained from New Zealand White rabbits, and the immunological identity of the enzyme from M. canis, as well as those from M. gypseum, T. mentagrophytes and T. rubrum, were demonstrated by immuno‐double‐diffusion experiments.