LABORATORY STUDIES OF COLLAGEN WOUND DRESSING (CAS)

E-ISSN： 1346-8138|11|3|253-258

ISSN： 0385-2407

Source： THE JOURNAL OF DERMATOLOGY, Vol.11, Iss.3, 1984-06, pp. : 253-258

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Abstract

ABSTRACTIn order to elucidate the behavior of collagen wound dressing (CAS) after application, the transport of radioactivity from 3H‐CAS applied to rabbit skin excision was determined.Neutral soluble collagen fraction was extracted from the skin of 30 rats given 30 mCi of 3H‐glycine, followed by crosslinking with glutaraldehyde. The resulting mixture was then used as a binder of unlabelled collagen web to form 3H‐CAS.Skin, 2.5 cm square and 0.02 inch deep, was excised with a dermatome on bilateral sites of the back of 6 rabbits.Then, 3H‐CAS sheet, 3.5 cm square, was applied to each excision with 20 pieces of gauze and an elastic bandage. Each animal was kept separately in a metabolic cage to collect urine and feces every 24 hours. After the animals were sacrificed on the 8th day, 3H‐CAS, gauze, elastic bandage, and the skin near the treatment area were collected, and the radioactivity was determined by a liquid scintillation counter after combustion in a sample oxidizer.More than 98.2% of radioactivity was recovered from 3H‐CAS after 8 days.Release of radioactivity from 3H‐CAS by exudate was negligible and percutaneous transport of collagen was not observed.No radioactivity was detected in rabbit serum, urine, or feces.