Microscopy Applied to Biophotonics ( International School of Physics “Enrico Fermi” )

Publication series ：International School of Physics “Enrico Fermi”

Author： Pavone   F.S.  

Publisher： Ios Press‎

Publication year： 2014

E-ISBN: 9781614994138

P-ISBN(Hardback):  9781614994121

Subject： O4 Physics

Keyword： 物理学

Language： ENG

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Description

Biophotonics and microscopy are highly inter-related fields in terms of both technological development and biomedical applications. Recent advances in microscopy have been paralleled by new opportunities for biophotonics, including the investigation and manipulation of biological phenomena using light and its application to biomedicine. This book contains papers from the Enrico Fermi International School of Physics on Microscopy Applied to Biophotonics, held in Varenna, Italy, in July 2011. The lectures spanned the basic science of imaging, through advanced microscopy techniques, to the state-of-the-art in biomedical imaging, and were complemented by seminars from world leaders in biophotonics. Subjects covered include: an overview of biophotonics; fundamentals of microscopy and an introduction to nonlinear microscopy; fluorescence; lasers for biophotonics; and an introduction to ultra-microscopy.

Chapter

Title Page

Title Page

Contents

Contents

Preface

Preface

Course group shot

Course group shot

Overview of fluorescence imaging for biophotonics

Overview of fluorescence imaging for biophotonics

Optical microscopes

Optical microscopes

Improved image quality and 3D imaging

Improved image quality and 3D imaging

Functional imaging using fluorescence

Functional imaging using fluorescence

Beyond the diffraction limit

Beyond the diffraction limit

High-speed and throughput

High-speed and throughput

Imaging in biological tissue

Imaging in biological tissue

Conclusions

Conclusions

Nonlinear imaging of tissues

Nonlinear imaging of tissues

Introduction

Introduction

Theoretical background

Theoretical background

Two-photon excitation fluorescence microscopy

Two-photon excitation fluorescence microscopy

Second-harmonic generation microscopy

Second-harmonic generation microscopy

Fluorescence lifetime imaging microscopy

Fluorescence lifetime imaging microscopy

Morphological imaging

Morphological imaging

Combined two-photon fluorescence?second-harmonic generation microscopy on skin tissue

Combined two-photon fluorescence?second-harmonic generation microscopy on skin tissue

Combined two-photon fluorescence?second-harmonic generation microscopy on diseased dermis tissue

Combined two-photon fluorescence?second-harmonic generation microscopy on diseased dermis tissue

Combined two-photon fluorescence?second-harmonic generation microscopy on bladder tissue

Combined two-photon fluorescence?second-harmonic generation microscopy on bladder tissue

Second-harmonic generation imaging on cornea

Second-harmonic generation imaging on cornea

Improving the penetration depth with two-photon imaging: application of Optical Clearing Agents

Improving the penetration depth with two-photon imaging: application of Optical Clearing Agents

Chemical imaging

Chemical imaging

Lifetime imaging of Basal Cell Carcinoma

Lifetime imaging of Basal Cell Carcinoma

Enhancing tumor margins with two-photon fluorescence by using aminolevulinic acid

Enhancing tumor margins with two-photon fluorescence by using aminolevulinic acid

Morpho-functional imaging

Morpho-functional imaging

Single spine imaging and ablation inside brain of small living animals

Single spine imaging and ablation inside brain of small living animals

Optical recording of electrical activity in intact neuronal network by random access second-harmonic (RASH) microscopy

Optical recording of electrical activity in intact neuronal network by random access second-harmonic (RASH) microscopy

Conclusion

Conclusion

New microscopy for the 3D visualization of large samples with highest resolution

New microscopy for the 3D visualization of large samples with highest resolution

Status of research of ultramicroscopy

Status of research of ultramicroscopy

3D visualization of fixed specimens

3D visualization of fixed specimens

Visualization of neurons

Visualization of neurons

Visualization of mouse embryos

Visualization of mouse embryos

Visualization of flies

Visualization of flies

Study of the development of the vascular system in the mouse embryo

Study of the development of the vascular system in the mouse embryo

High dynamic range imaging

High dynamic range imaging

Raman spectroscopy: A powerful tool for biomedical applications

Raman spectroscopy: A powerful tool for biomedical applications

Introduction and theoretical background

Introduction and theoretical background

Results and discussions

Results and discussions

Biomedical SERS approaches

Biomedical SERS approaches

Rapid identification of microorganisms

Rapid identification of microorganisms

Cell and tissue diagnostics via linear and non-linear Raman imaging

Cell and tissue diagnostics via linear and non-linear Raman imaging

Conclusion

Conclusion

In vivo optical flow cytometry and cell imaging

In vivo optical flow cytometry and cell imaging

Goal and motivation

Goal and motivation

Introduction

Introduction

In vitro conventional flow cytometry

In vitro conventional flow cytometry

Intravital video microscopy

Intravital video microscopy

Animal models

Animal models

In vivo fluorescence flow cytometry (IVFFC)

In vivo fluorescence flow cytometry (IVFFC)

Single-photon intravital fluorescence flow cytometry (SPIVFFC)

Single-photon intravital fluorescence flow cytometry (SPIVFFC)

Multiphoton intravital fluorescence flow cytometry (MPIVFFC)

Multiphoton intravital fluorescence flow cytometry (MPIVFFC)

Label-free back-reflectance in vivo cytometry

Label-free back-reflectance in vivo cytometry

In vivo cytometry based on transmittance digital microscopy (TDM)

In vivo cytometry based on transmittance digital microscopy (TDM)

Basic principles

Basic principles

High-speed, high-resolution in vivo imaging cytometry

High-speed, high-resolution in vivo imaging cytometry

Instrumentation and some applications

Instrumentation and some applications

Optical clearing for in vivo label-free image cytometry

Optical clearing for in vivo label-free image cytometry

Cell velocity measurements using TDM

Cell velocity measurements using TDM

Summary

Summary

Photothermal (PT) and photoacoustic (PA) flow cytometry techniques

Photothermal (PT) and photoacoustic (PA) flow cytometry techniques

Fundamentals PT and PA techniques

Fundamentals PT and PA techniques

Integrated photothermal cytometry in vivo

Integrated photothermal cytometry in vivo

PT velocimetry: principle

PT velocimetry: principle

Principles of laser-based PT detection of nano-scaled objects

Principles of laser-based PT detection of nano-scaled objects

Photoacoustic studies of blood and lymph flows

Photoacoustic studies of blood and lymph flows

PA/PT lymph and blood flow cytometry and cancer cell ablation

PA/PT lymph and blood flow cytometry and cancer cell ablation

Conclusion

Conclusion

Resonant nonlinear optical microscopy

Resonant nonlinear optical microscopy

Introduction

Introduction

Vibrationally resonant non-linear optical microscopy

Vibrationally resonant non-linear optical microscopy

Vibrational modes of molecules

Vibrational modes of molecules

Infrared absorbtion

Infrared absorbtion

Raman Scattering (RS)

Raman Scattering (RS)

Coherent Raman Scattering (CRS)

Coherent Raman Scattering (CRS)

CARS and CSRS

CARS and CSRS

SRL and SRG

SRL and SRG

Maximum signal and sensitivity

Maximum signal and sensitivity

Excitation of CRS

Excitation of CRS

Single-frequency excitation

Single-frequency excitation

Single-time excitation

Single-time excitation

Multiplex excitation

Multiplex excitation

Conclusion

Conclusion

Imaging absorbers with small quantum yield

Imaging absorbers with small quantum yield

Transient four-wave mixing of quantum dots: theory background

Transient four-wave mixing of quantum dots: theory background

Four-wave mixing imaging of quantum dots

Four-wave mixing imaging of quantum dots

Transient four-wave mixing dynamics of gold nanoparticles: theory and experiments

Transient four-wave mixing dynamics of gold nanoparticles: theory and experiments

Four-wave mixing imaging of gold nanoparticles

Four-wave mixing imaging of gold nanoparticles

Summary and outlook

Summary and outlook

Posters

Posters

Polarisation-in polarisation-out second-harmonic generation microscopy in birefringent biological structures

Polarisation-in polarisation-out second-harmonic generation microscopy in birefringent biological structures

Introduction and theory

Introduction and theory

Methods and materials

Methods and materials

Results and discussion

Results and discussion

Co-integration of microfluidic and optical functions for analysis in nuclear environment

Co-integration of microfluidic and optical functions for analysis in nuclear environment

Introduction

Introduction

Integrated optics and ion-exchange on glass

Integrated optics and ion-exchange on glass

Absorption optical sensor

Absorption optical sensor

Increasing the interaction length: use of segmented waveguides

Increasing the interaction length: use of segmented waveguides

Trapping of light: cavity enhanced spectroscopy

Trapping of light: cavity enhanced spectroscopy

Device under investigation

Device under investigation

Conclusion and perspectives

Conclusion and perspectives

Automated classification of healthy and keloidal collagen patterns based on processing of SHG images of human skin

Automated classification of healthy and keloidal collagen patterns based on processing of SHG images of human skin

Introduction

Introduction

Image recording

Image recording

Image processing and analysis

Image processing and analysis

Conclusion

Conclusion

List of participants

List of participants

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