Dynamics of Leaf Photosynthesis :Rapid Response Measurements and Their Interpretations

Publication subTitle :Rapid Response Measurements and Their Interpretations

Author: Laisk A; Oja V  

Publisher: CSIRO PUBLISHING‎

Publication year: 1998

E-ISBN: 9780643105294

P-ISBN(Paperback): 9780643063785

Subject: Q945.11 photosynthesis carbon assimilation

Keyword: 植物学

Language: ENG

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Description

Details a novel approach to dynamic, as opposed to steady-state, analysis of leaf photosynthesis.

Chapter

2.5 Design of the rapid-response leaf chamber with contact thermostatting

2.6 Irradiation sources and fibre optics

2.7 Gas analysis

2.7.1 Carbon dioxide

2.7.2 Water vapour

2.7.3 Oxygen

2.8 Data logging and processing

2.9 Movement of the gas front through the leaf chamber and whole-system response

2.10 Modified rapid-response system for CO[sub(2)] titration of leaves using changes in O[sub(2)] concentration

3 Kinetic analysis of leaf carbon metabolism in steady-state C[sub(3)] photosynthesis

3.1 Calculation of the effective CO[sub(2)] concentration during photosynthesis in leaves

3.1.1 The network of condensed diffusion resistances and fluxes in a leaf

3.1.2 Expression of fluxes using the difference of the chemical potential of CO[sub(2)]

3.2 Dependence of the calculated intercellular CO[sub(2)] concentrations on the statistical distribution of stomatal apertures

3.3 The effect of leaf optical density on the shape of light-response curves

3.4 Determining photosynthetic parameters from steady-state CO[sub(2)] exchange measurements

3.4.1 Theoretical relationships between electron transport, CO[sub(2)] fixation and photorespiratory CO[sub(2)] evolution

3.4.2 CO[sub(2)]/O[sub(2)] specificity of rubisco

3.4.3 Dark respiration in the light

3.4.4 Mesophyll diffusion resistance

3.5 From steady-state to transient analysis of leaf CO[sub(2)] uptake

3.5.1 Steady-state and transient CO[sub(2)] curves

3.5.2 Steady-state and transient light curves

4 Investigations of transient processes in C[sub(3)] leaf photosynthesis

4.1 Dark-light induction of photosynthesis

4.1.1 CO[sub(2)] uptake during the dark-light induction of photosynthesis

4.1.2 O[sub(2)] evolution during the induction under light saturation and light limitation

4.1.3 Simultaneous measurements of CO[sub(2)] uptake and O[sub(2)] evolution during induction

4.2 Kinetic studies of photosynthetic carboxylation in vivo

4.2.1 Kinetics of rubisco with respect to CO[sub(2)]

4.2.2 Post-illumination CO[sub(2)] uptake: kinetics of rubisco in vivo with respect to RuBP

4.2.3 Analysing the inhibition of rubisco in leaves by post-illumination transients

4.3 Measurement of photorespiratory CO[sub(2)] evolution in the light

4.4 Application of rapid-response O[sub(2)] exchange measurements in studies of electron transport kinetics

4.4.1 Indicators of the intermediate pools in the electron transport chain

4.4.2 Improving the time resolution of O[sub(2)] evolution measurements: the pulse method

4.4.3 Measurement of the light-response curve of PS II

5 Investigations of proton concentration and transport in leaves by gas exchange methods

5.1 CO[sub(2)] capacity of leaf as an indicator of the pH in chloroplast stroma

5.2 CO[sub(2)] titration of leaves

6 Probing PS II in vivo using rapid-response gas exchange and chlorophyll fluorescence

6.1 Physical background of chlorophyll fluorescence in leaves

6.2 Simple theory of fluorescence: expressions for quantum yields and rate constants

6.3 Measurement of the quantum yield of fluorescence

6.4 Comparison of electron transport rates calculated from fluorescence and gas exchange

6.5 Thermoinhibition of photosynthesis as analysed by chlorophyll fluorescence and CO[sub(2)] exchange

6.6 Photoinhibition of photosynthesis as analysed by chlorophyll fluorescence and O[sub(2)] evolution

6.7 Relationship between quantum yields of O[sub(2)] evolution and fluorescence at constant non-photochemical quenching

6.8 Quantum yields and rate constants of photochemical and non-photochemical excitation quenching under 'energized' and inhibitory conditions.

6.9 Light-response curves of PS II electron transport in the absence and in the presence of non-photochemical excitation quenching

6.10 About the mechanism of non-photochemical excitation quenching

7 Probing PS I in vivo using rapid-response gas exchange and absorbance around 800 nm

7.1 Electron and excitation budget at PS I

7.2 Absorbance at 800 nm as a probe for P700 reduction state in leaves

7.3 Techniques of measurement of 800 nm absorbance changes

7.4 Calibration of the 800 nm signal for complete reduction and oxidation of P700

7.4.1 The 800 nm signal in the dark and in light pulses

7.4.2 The 800 nm signal under far-red light (FRL)

7.5 Quantum yield of PS I from interpretation of the steady-state 800 nm signal

7.6 Control of PS I electron transport by acceptor side reduction and excitation capture efficiency, deduced from the 800 nm signal

7.7 The kinetics of photosynthetic control measured by the post-illumination re-reduction of P700

7.8 PS I acceptor side control during dark–light induction and oscillations of photosynthesis

8 Concluding remarks

References

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