CONTENTS

PREFACE

List of Contributors

Medical Diagnosis

Magnetic Resonance Spectroscopy and Imaging in Breast Cancer Prognosis and Diagnosis

INTRODUCTION

NMR-based Metabolomics Approaches

Magnetic Resonance Spectroscopy (MRS)

Magnetic Resonance Imaging (MRI)

APPLICATIONS OF MAGNETIC RESONANCE TECHNIQUES TO BREAST CANCER RESEARCH

Applications of NMR-Based Metabolomics to Breast Cancer Research

High-Resolution Magic Angle Spinning Magnetic Resonance Spectroscopy (HR-MAS MRS)

In vivo Magnetic Resonance Spectroscopy (MRS)

Magnetic Resonance Imaging

Magnetic Resonance Detection Limit in Breast Cancer

DISCUSSION AND CONCLUSION

CONFLICT OF INTEREST

ACKNOWLEDGMENTS

REFERENCES

Food Sciences

Application of NMR Spectroscopy for the Characterization of Dietary Polyphenols

INTRODUCTION

NMR SEPCTROSCOPY

Technical Aspects for NMR Measurement

2-Dimensional NMR

NMR Coupled to Other Analytical Instruments

POLYPHENOLS

Flavonoids

Anthocyanins

Tannins

APPLICATIONS

Tea

Grape and Wine

Cocoa and Chocolate

Others

SUMMARY

CONFLICT OF INTEREST

ACKNOWLEDGEMENTS

REFERENCES

Molecular Identification (Secondary Metabolites)

New Developments in NMR Methodologies with Special Roles in Natural Product Discovery

INTRODUCTION

NMR INSTRUMENTATION ADVANCEMENTS: HIGH FIELD MAGNETS AND INNOVATIVE PROBES

Increased Magnetic Field Strengths

NMR Probe Miniaturization

Cryogenic NMR Spectroscopy

Application of Advances in NMR Instrumentation Toward Natural Product Drug Discovery

HYPHENATED NMR METHODS

HPLC-NMR and Related Techniques

GC-NMR

CE-NMR and Related Techniques

DIRECT NMR ANALYSES OF METABOLITES WITHIN COMPLEX MIXTURES AND METABOLOMICS

Metabolomics

PERSPECTIVE AND CONCLUSION

CONFLICT OF INTEREST

ACKNOWLEDGEMENTS

REFERENCES

Investigations of Biomolecular Conformation and Dynamics using 19F NMR

INTRODUCTION

19F LABELING STRATEGIES IN BIOMOLECULES

PROTEIN APPLICATIONS

PROTEIN STRUCTURE

PROTEIN DYNAMICS

PROTEIN FOLDING AND AGGREGATION

AMYLOID FORMATION

BINDING STUDIES

PROTEIN-BIOMOLECULAR INTERACTIONS

IN VIVO PROTEIN STRUCTURE

FLUORINATED NUCLEOSIDES AND OLIGOMERS

FLUOROCARBON AND NUCLEOSIDE AMPIPHILES

DNA/RNA SECONDARY STRUCTURE

NUCLEIC ACIDS DYNAMICS

CONCLUSION

CONFLICT OF INTEREST

ACKNOWLEDGEMENTS

REFERENCES

NMR of the Enaminones

INTRODUCTION

HISTORY

Why Use Nuclear Magnetic Resonance (NMR) Spectroscopy?

MOLECULAR MODELING

Computational NMR

NMR OF THE ENAMINONES

Sulfonamide Enaminones

CONCLUSION

CONFLICT OF INTEREST

ACKNOWLEDGEMENTS

REFERENCES

Molecular Identification (Structural Biology)

Monitoring Intermolecular and Intramolecular Interactions by NMR Spectroscopy

INTRODUCTION

NMR For Monitoring Intermolecular and Intramolecular Interactions

NMR Methods Observing Macromolecular Targets

NMR Methods That Observe Ligands

In vivo methods

NMR Metabolomics

Sampling

NMR Spectroscopy in Metabolomics

Data Analysis

Metabolomics in diagnosis of Human Diseases and Disorders

Plant Metabolomics

CONCLUSION

CONFLICT OF INTEREST

AKNOWLEDGEMENTS

REFERENCES

SUBJECT INDEX