Characterization of ACC deaminase from the biocontrol and plant growth-promoting agent Trichoderma asperellum T203

Author： Viterbo Ada Landau Udi Kim Sofia Chernin Leonid Chet Ilan

ISSN： 0378-1097

Source： FEMS Microbiology Letters, Vol.305, Iss.1, 2010-04, pp. : 42-48

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Abstract

Abstract1-Aminocyclopropane-1-carboxylate (ACC) deaminase activity was evaluated in the biocontrol and plant growth-promoting fungus Trichoderma asperellum T203. Fungal cultures grown with ACC as the sole nitrogen source showed high enzymatic activity. The enzyme encoding gene (Tas-acdS) was isolated, and an average 3.5-fold induction of the gene by 3 mM ACC was detected by real-time PCR. Escherichia coli bacteria carrying the intron-free cDNA of Tas-acdS cloned into the vector pAlter-EX1 under the control of the tac promoter revealed specific ACC deaminase (ACCD) activity and the ability to promote canola (Brassica napus) root elongation in pouch assays. RNAi silencing of the ACCD gene in T. asperellum showed decreased ability of the mutants to promote root elongation of canola seedlings. These data suggest a role for ACCD in the plant root growth-promotion effect by T. asperellum.