Cloning and restriction analysis of ribosomal RNA genes from

Author： Bercovier H.   Kafri O.   Kornitzer D.   Sela S.  

Publisher： Blackwell Publishing

ISSN： 0378-1097

Source： FEMS Microbiology Letters, Vol.57, Iss.2, 1989-01, pp. : 125-128

Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.

Previous Menu Next

Abstract

Abstract A genomic library of Mycobacterium smegmatis DNA was constructed in phage EMBL3. A clone (λ HB85) containing rRNA genes was isolated using as probes, fragments of E. coli rRNA cistron B. This cloned DNA fragment was mapped by restriction analysis and was shown to contain one complete set of rRNA genes (rRNA A). The physical mapping of the second set of rRNA genes of M. smegmatis (rRNA B) was done by restriction analysis of total chromosomal DNA. The two sets of rRNA genes showed highly conserved restriction sites within the respective sets but not in the flanking regions. The two rRNA sets of genes are organised like in the other eubacteria in the order 16S-23S-5S.

Related content

Sequence analysis of 16S rRNA genes amplified from two ribosomal RNA gene clusters of Bifidobacterium bifidum

By Yoon J-H.  

Antonie van Leeuwenhoek, Vol. 75, Iss. 4, 1999-05 ,pp. : 329-333 (5)

Springer Publishing Company

Access to resources Recommend Favorite

Phylogeny of the Genus Agaricus Inferred from Restriction Analysis of Enzymatically Amplified Ribosomal DNA

By Bunyard B.A.   Nicholson M.S.   Royse D.J.  

Fungal Genetics and Biology, Vol. 20, Iss. 4, 1996-12 ,pp. : 243-253 (11)

Academic Press

Access to resources Recommend Favorite

Taxonomical classification of yeasts isolated from kefir based on the sequence of their ribosomal RNA genes

By Latorre-García Lorena   Castillo-Agudo Lucas   Polaina Julio  

World Journal of Microbiology and Biotechnology, Vol. 23, Iss. 6, 2007-06 ,pp. : 785-791 (7)

Springer Publishing Company

Access to resources Recommend Favorite

Advances in the use of terminal restriction fragment length polymorphism (T-RFLP) analysis of 16S rRNA genes to characterize microbial communities

By Schütte Ursel  

Applied Microbiology and Biotechnology, Vol. 80, Iss. 3, 2008-09 ,pp. : 365-380 (16)

Springer Publishing Company

Access to resources Recommend Favorite

Effect of restriction endonucleases on assessment of biodiversity of cultivable polar marine planktonic bacteria by amplified ribosomal DNA restriction analysis

By Zeng Yinxin   Liu Wenqi   Li Huirong   Yu Yong   Chen Bo  

Extremophiles, Vol. 11, Iss. 5, 2007-09 ,pp. : 685-692 (8)

Springer Publishing Company

Access to resources Recommend Favorite