Chloroquine enhances replication of influenza A virus A/WSN/33 (H1N1) in dose‐, time‐, and MOI‐dependent manners in human lung epithelial cells A549

Publisher: John Wiley & Sons Inc

E-ISSN: 1096-9071|87|7|1096-1103

ISSN: 0146-6615

Source: JOURNAL OF MEDICAL VIROLOGY, Vol.87, Iss.7, 2015-07, pp. : 1096-1103

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Abstract

Anti‐malaria drug, chloroquine, has been reported to be effective against influenza A virus (IAV) in vitro and used in in‐vivo experiments and clinical trial for prevention or treatment of influenza. In this study, it has been shown by immunofluorescence, hemagglutination, and plaque assays that chloroquine enhanced A/WSN/33 (H1N1) replication with pronounced cytopathic effect in dose‐, time‐, and MOI‐dependent manners in human lung epithelial cells A549. Time‐of‐addition assay showed that inhibitory effect on virus replication by chloroquine pre‐treatment was indistinctive, and virus productions were enhanced when the drug was applied after viral adsorption. The effectiveness of chloroquine as an anti‐influenza drug is questioned, and caution in its use is recommended. J. Med. Virol. 87:1096–1103, 2015. © 2015 Wiley Periodicals, Inc.

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